Botulinum neurotoxin type C1 Antibody, FITC conjugated

^{Rare Species}

說明書

中文名稱：

Botulinum neurotoxin type C1兔多克隆抗體, FITC偶聯
貨號：

CSB-PA325596LC01CLQ
規格：

￥880
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

產品名稱：

Rabbit anti-Clostridium botulinum N/A Polyclonal antibody
Uniprot No.：

P18640
基因名：

N/A
別名：

Botulinum neurotoxin type C antibody; BoNT/C antibody; Bontoxilysin-C1 antibody; BoNT/C1 antibody; Botulinum neurotoxin type C1) [Cleaved into: Botulinum neurotoxin C light chain antibody; LC antibody; EC 3.4.24.69); Botulinum neurotoxin C heavy chain antibody; HC)] antibody
宿主：

Rabbit
反應種屬：

Clostridium botulinum
免疫原：

Recombinant Clostridium botulinum Botulinum neurotoxin type C1 protein (2-428AA)
免疫原種屬：

Clostridium botulinum
標記方式：

FITC
克隆類型：

Polyclonal
抗體亞型：

IgG
純化方式：

>95%, Protein G purified
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
產品提供形式：

Liquid
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Botulinum toxin causes flaccid paralysis by inhibiting neurotransmitter (acetylcholine) release from the presynaptic membranes of nerve terminals of the eukaryotic host skeletal and autonomic nervous system, with frequent heart or respiratory failure. Is unique among characterized BoNTs in having 2 substrates, syntaxin (STX) and SNAP25. Precursor of botulinum neurotoxin C which unlike most BoNTs seems not to have a proteinaceous coreceptor, and instead recognizes 2 different complex polysialylated gangliosides found on neural tissue probably found in synaptic vesicles. Upon synaptic vesicle recycling the toxin is taken up via the endocytic pathway. When the pH of the toxin-containing endosome drops a structural rearrangement occurs so that the N-terminus of the heavy chain (HC) forms pores that allows the light chain (LC) to translocate into the cytosol. Once in the cytosol the disulfide bond linking the 2 subunits is reduced and LC cleaves its target protein on synaptic vesicles, preventing their fusion with the cytoplasmic membrane and thus neurotransmitter release. In vitro the whole toxin only has protease activity after reduction. Electrical stimulation increases uptake of toxin, presumably by transiently exposing a receptor usually found in eukaryotic target synaptic vesicles. Forms ion-conducting channels at around pH 6.1. Requires complex eukaryotic host polysialogangliosides for full neurotoxicity. Synaptic vesicle glycoproteins (SV2) do not seem to act as its receptor.; Has proteolytic activity. After translocation into the eukaryotic host cytosol, inhibits neurotransmitter release by acting as a zinc endopeptidase that cleaves syntaxin-1A/STX1A and syntaxin-1B/STX1B. Cleaves the '253-Arg-|-Ala-254' bond of STX1 and the '252-Arg-|-Ala-253' bond of STX2; also acts on syntaxin 3 (STX3) but not 4 (STX4). Cleaves the '198-Arg-|-Ala-199' bond of SNAP25. Recognizes the '93-Asn--Met-202' region of SNAP25.; Responsible for host epithelial cell transcytosis, host nerve cell targeting and translocation of light chain (LC) into eukaryotic host cytosol. Composed of 3 subdomains; the translocation domain (TD), and N-terminus and C-terminus of the receptor-binding domain (RBD). The RBD is responsible for the adherence of the toxin to the eukaryotic target cell surface. It simultaneously recognizes 2 polysialated gangliosides coreceptors in close proximity on host synaptic vesicles. The N-terminus of the TD wraps an extended belt around the perimeter of the LC, protecting Zn(2+) in the active site; it may also prevent premature LC dissociation from the translocation channel and protect toxin prior to translocation. The TD inserts into synaptic vesicle membrane to allow translocation into the host cytosol. The C-terminal half of the HC (residues 864-1291) binds neurons in a dose-dependent manner. The C-terminal half of the HC (residues 863-1291) binds eukaryotic host gangliosides in the order GD1b > GT1b > GD1a > GM1a. Has 2 ganglioside binding sites; Sia-1 prefers a sia7 sialic acid and sugars within the ganglioside (GD1b > GT1b), whereas GBP2 recognizes a sia5 sialic acid (GT1b and GD1a). Both sites are required for HC to enter neurons, acting via different gangliosides. This suggests that 2 gangliosides serve as toxin receptors. Synaptic activity (depolarization with K(+)) increases uptake by neurons. Treatment of synaptosomes with proteinase K does not reduce HC binding, suggesting there is no protein receptor or it is protected from extracellular proteases. Decreases uptake and toxicity of whole BoNT/A, but also interferes with uptake of BoNT/E and BoNT/F. HC also binds phosphoinositides, which might play a role in membrane-binding.
亞細胞定位：

[Botulinum neurotoxin type C]: Secreted.; [Botulinum neurotoxin C light chain]: Secreted.; [Botulinum neurotoxin C heavy chain]: Secreted.
蛋白家族：

Peptidase M27 family

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