ATG4A Antibody

說明書

中文名稱：

ATG4A兔多克隆抗體
貨號：

CSB-PA438009
規格：

￥1100
圖片：
The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using CSB-PA438009(ATG4A Antibody) at dilution 1/45, on the right is treated with synthetic peptide. (Original magnification: ×200)

The image on the left is immunohistochemistry of paraffin-embedded Human gastric cancer tissue using CSB-PA438009(ATG4A Antibody) at dilution 1/45, on the right is treated with synthetic peptide. (Original magnification: ×200)

Gel: 10%SDS-PAGE, Lysate: 40 μg, Lane: 231 cells, Primary antibody: CSB-PA438009(ATG4A Antibody) at dilution 1/210, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 2 minutes
其他：

產品詳情
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靶點詳情

產品詳情

Uniprot No.：

Q8WYN0
基因名：

ATG4A
別名：

AI627006 antibody; APG4 autophagy 4 homolog A antibody; Apg4a antibody; ATG4 autophagy related 4 homolog A (S. cerevisiae) antibody; ATG4 autophagy related 4 homolog A antibody; ATG4A antibody; ATG4A_HUMAN antibody; Atg4al antibody; AUT like 2 cysteine endopeptidase antibody; AUT-like 2 cysteine endopeptidase antibody; Autl2 antibody; Autophagin 2 antibody; Autophagin-2 antibody; Autophagy related 4A cysteine peptidase antibody; Autophagy related cysteine endopeptidase 2 antibody; Autophagy related protein 4 homolog A antibody; Autophagy-related cysteine endopeptidase 2 antibody; Autophagy-related protein 4 homolog A antibody; AV169859 antibody; Cysteine protease ATG4A antibody; hAPG4A antibody; MGC107179 antibody
宿主：

Rabbit
反應種屬：

Human
免疫原：

Synthetic peptide of Human ATG4A
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
抗體亞型：

IgG
純化方式：

Antigen affinity purification
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

-20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
產品提供形式：

Liquid
應用范圍：

ELISA,WB,IHC

推薦稀釋比：

Application	Recommended Dilution
ELISA	1:1000-1:5000
WB	1:200-1:1000
IHC	1:50-1:200

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunohistochemistry (IHC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Cleaves the C-terminal amino acid of ATG8 family proteins to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Preferred substrate is GABARAPL2 followed by MAP1LC3A and GABARAP. Has also an activity of delipidating enzyme for the PE-conjugated forms.
基因功能參考文獻：
1. An intron SNP rs807185 in ATG4A might be a protective factor for lung cancer. PMID: 26061994
2. ATG4A promotes the metastasis of gastric cancer cells via the Notch signaling pathway, which is an autophagy-independent mechanism PMID: 27276686
3. The structure and regulatory machinery of Atg4a [review] PMID: 28253956
4. We conclude that miR-24-3p regulates autophagy by targeting ATG4A PMID: 25426560
5. The autophagy regulator ATG4A was found to be essential for the maintenance of a sub-population with cancer stem cell properties and to regulate breast cancer cell tumourigenicity. PMID: 24229464
6. The demonstration that expression of ATG4A in cells increased stem properties provided an indication of its biological function. Hypomethylation of ATG4A and HIST1H2BN in ovarian tumor-initiating cells predicts poor prognosis for ovarian cancer patients. PMID: 24256813
7. no association between SNP rs807185 and the presence of granulomas in Crohn's disease PMID: 21122541
8. statistical analysis of APG4A gene confirmed the characterisation of each protein-coding gene present in human genome Xq22.1 PMID: 21297227
9. Atg4B possessed the broadest spectrum against all substrates, followed by Atg4A for ATG8 substrates PMID: 21177865
10. HsApg4A cleaves the COOH terminus of GATE-16 PMID: 12473658
11. The cysteine protease HsAtg4 is identified as a direct target for oxidation by hydrogen peroxide, and a cysteine residue located near the HsAtg4 catalytic site as critical for this regulation. PMID: 17347651
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亞細胞定位：

Cytoplasm.
蛋白家族：

Peptidase C54 family
數據庫鏈接：

HGNC: 16489

OMIM: 300663

KEGG: hsa:115201

STRING: 9606.ENSP00000361306

UniGene: Hs.8763

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