ALG11 Antibody

說(shuō)明書

中文名稱：

ALG11兔多克隆抗體
貨號(hào)：

CSB-PA647192LA01HU
規(guī)格：

￥440
圖片：
IHC image of CSB-PA647192LA01HU diluted at 1:250 and staining in paraffin-embedded human adrenal gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunofluorescence staining of HepG2 cells with CSB-PA647192LA01HU at 1:105, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
其他：

產(chǎn)品詳情

產(chǎn)品名稱：

Rabbit anti-Homo sapiens (Human) ALG11 Polyclonal antibody
Uniprot No.：

Q2TAA5
基因名：

ALG11
別名：

ALG11; GT8; GDP-Man:Man(3GlcNAc(2-PP-Dol alpha-1,2-mannosyltransferase; Asparagine-linked glycosylation protein 11 homolog; Glycolipid 2-alpha-mannosyltransferase
宿主：

Rabbit
反應(yīng)種屬：

Human
免疫原：

Recombinant Human GDP-Man:Man(3)GlcNAc(2)-PP-Dol alpha-1,2-mannosyltransferase protein (416-480AA)
免疫原種屬：

Homo sapiens (Human)

標(biāo)記方式：

Non-conjugated

本頁(yè)面中的產(chǎn)品，ALG11 Antibody (CSB-PA647192LA01HU)，的標(biāo)記方式是Non-conjugated。對(duì)于ALG11 Antibody，我們還提供其他標(biāo)記。見下表：

標(biāo)記方式	貨號(hào)	產(chǎn)品名稱	應(yīng)用
HRP	CSB-PA647192LB01HU	ALG11 Antibody, HRP conjugated	ELISA
FITC	CSB-PA647192LC01HU	ALG11 Antibody, FITC conjugated
Biotin	CSB-PA647192LD01HU	ALG11 Antibody, Biotin conjugated	ELISA

克隆類型：

Polyclonal
抗體亞型：

IgG
純化方式：

>95%, Protein G purified
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
產(chǎn)品提供形式：

Liquid
應(yīng)用范圍：

ELISA, IHC, IF

推薦稀釋比：

Application	Recommended Dilution
IHC	1:200-1:500
IF	1:50-1:200

Protocols：

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
儲(chǔ)存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

功能：

Mannosyltransferase involved in the last steps of the synthesis of Man5GlcNAc(2)-PP-dolichol core oligosaccharide on the cytoplasmic face of the endoplasmic reticulum. Catalyzes the addition of the 4th and 5th mannose residues to the dolichol-linked oligosaccharide chain.
基因功能參考文獻(xiàn)：
1. Hence, we concluded that there is different transcriptional control mechanism between mALG11 and hALG11 PMID: 25036826
2. After identifying the congenital disorders of glycosylation-Ip index patient, study describe three more cases suffering from an ALG11 deficiency. PMID: 22213132
3. Deficiency of the endoplasmatic mannosyltransferase hALG11 leads to congenital disorder of glycosylation. PMID: 20080937
相關(guān)疾病：

Congenital disorder of glycosylation 1P (CDG1P)
亞細(xì)胞定位：

Endoplasmic reticulum. Endoplasmic reticulum membrane; Multi-pass membrane protein.
蛋白家族：

Glycosyltransferase group 1 family, Glycosyltransferase 4 subfamily
數(shù)據(jù)庫(kù)鏈接：

HGNC: 32456

OMIM: 613661

KEGG: hsa:440138

STRING: 9606.ENSP00000430236

UniGene: Hs.512963

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