ACHE Antibody

中文名稱：

ACHE兔多克隆抗體
貨號：

CSB-PA5619A0Rb
規(guī)格：

￥440
圖片：
IHC image of CSB-PA5619A0Rb diluted at 1:300 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunofluorescence staining of MCF-7 cells with CSB-PA5619A0Rb at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
其他：

產(chǎn)品詳情
產(chǎn)品評價
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靶點詳情

產(chǎn)品詳情

產(chǎn)品名稱：

Rabbit anti-Homo sapiens (Human) ACHE Polyclonal antibody
Uniprot No.：

P22303
基因名：

ACHE
別名：

ACEE antibody; ACES_HUMAN antibody; Acetylcholinesterase antibody; AChE antibody; Apoptosis related acetylcholinesterase antibody; ARACHE antibody; N ACHE antibody; N-ACHE antibody; YT antibody; YT blood group antibody
宿主：

Rabbit
反應種屬：

Human
免疫原：

Recombinant Human Acetylcholinesterase protein (53-162AA)
免疫原種屬：

Homo sapiens (Human)

標記方式：

Non-conjugated

本頁面中的產(chǎn)品，ACHE Antibody (CSB-PA5619A0Rb)，的標記方式是Non-conjugated。對于ACHE Antibody，我們還提供其他標記。見下表：

標記方式	貨號	產(chǎn)品名稱	應用
HRP	CSB-PA5619B0Rb	ACHE Antibody, HRP conjugated	ELISA
FITC	CSB-PA5619C0Rb	ACHE Antibody, FITC conjugated
Biotin	CSB-PA5619D0Rb	ACHE Antibody, Biotin conjugated	ELISA

克隆類型：

Polyclonal
抗體亞型：

IgG
純化方式：

>95%, Protein G purified
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
產(chǎn)品提供形式：

Liquid
應用范圍：

ELISA, IHC, IF

推薦稀釋比：

Application	Recommended Dilution
IHC	1:200-1:500
IF	1:50-1:200

Protocols：

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評價

靶點詳情

功能：

Terminates signal transduction at the neuromuscular junction by rapid hydrolysis of the acetylcholine released into the synaptic cleft. Role in neuronal apoptosis.
基因功能參考文獻：
1. 1-Naphthyl acetate (1-NA) to be a better alternative substrate for AChE than ATCh in terms of lower Km value. Its specificity appeared at least similar to ATCh. Therefore, we propose that 1-NA can be an attractive chromogenic substrate for the measurement of AChE activity. PMID: 30201403
2. AChE polymorphism was significantly associated to reduced activity in both multiple sclerosis patients and controls. PMID: 29358722
3. this study concludes and confirms that the aryl acylamidase activity of AChE is actively involved in the process of osteoblast differentiation and mineralization. PMID: 28852920
4. The activity of human erythrocyte acetylcholinesterase differs in males and females and serves as a biomarker for diverse range of diseases. (Review) PMID: 28885588
5. These findings suggest that, during Red Blood Cell ageing, GPI-linked proteins and integral membrane proteins are differentially sorted. Also, the vesicles that are generated in vitro show a fast and extensive loss of AChE activity, but not of AChE expression. PMID: 28518050
6. Our analysis showed that DMSO is a considerably potent and highly selective irreversible mixed-competitive inhibitor of human AChE with IC50 values in the lower millimolar range, corresponding to 0.88% to 2.6% DMSO (v/v). Most importantly, 1-4% (v/v) DMSO, the commonly used experimental concentrations, showed approximately 37-80% inhibition of human AChE activity. PMID: 29017007
7. This study found an increase in the protein and transcript levels of the non-cholinergic "readthrough" AChE (AChE-R) variants in Alzheimer's Disease patients compared to controls. PMID: 27258420
8. The optimized docking protocol was validated by an external test set of 11 natural galantamine derivatives and the correlation coefficient between the docking scores and the pIC50 values was 0.800. The derived relationship was used to analyze the interactions between galantamine derivatives and AChE. PMID: 27490385
9. These six-membered carbocycles showed nice inhibitory action against AChE and human carbonic anhydrase (hCA) II and I isoforms. The hCA I, II, and AChE were efficiently inhibited by these molecules, with Ki values in the range of 6.70-35.85 nM for hCA I, 18.77-60.84 nM for hCA II, and 0.74-4.60 for AChE, respectively PMID: 28613396
10. Discovery of potent carbonic anhydrase, acetylcholinesterase, and butyrylcholinesterase enzymes inhibitors: The new amides and thiazolidine-4-ones synthesized on an acetophenone base.() PMID: 28544359
11. hnRNP H binds to two specific G-runs in exon 5a of ACHE and activates the distal alternative 3 splice site (ss) between exons 5a and 5b. Furthermore, hnRNP H competes for binding of CstF64 to the overlapping binding sites in exon 5a, and suppresses the selection of a cryptic polyadenylation site, which additionally ensures transcription of the distal 3 ss required for the generation of AChET isoform. PMID: 28180311
12. these results suggest that the design and investigation of multifunctional agents containing along with the acetylcholinesterase inhibitory segment also an antioxidant moiety capable of alleviating metal (copper)-induced oxidative stress, may be of importance in the treatment of Alzheimer's disease. PMID: 27230386
13. Significance of AChE Genetic Variants to Risk of Toxicity from Cholinesterase Inhibitors (review) PMID: 27551784
14. miR-124 could directly target the 3'-untranslated region of both signal transducer and activator of transcription 3 (STAT3) and acetylcholinesterase (AChE) mRNAs, and suppress their protein expressions. PMID: 27977009
15. AChE activity in smokers was elevated (approx. 3% in males; 8% in females) relative to that in non-smokers. PMID: 28465191
16. Unusually high AChE activity may be an effect marker of exposure to ethanol. The relationship between AChE and apoptosis might represent a novel mechanism of ethanol-associated neuronal injury. PMID: 28427893
17. Thus C-547 is one of the most potent and selective reversible inhibitors of AChE with a long residence time, tau=20 min, longer than for other reversible inhibitors used in the treatment of myasthenia gravis. This makes C-547 a promising drug for the treatment of this disease PMID: 26929400
18. Studies have demonstrated involvement of inherited tendencies of AChE increases in response to stress. PMID: 27138800
19. Data suggest membranes of erythrocytes of patients with chronic obstructive pulmonary disease exhibit the following changes: increase in acetylcholinesterase; decrease in total ATPases and Na+/K+-ATPases; increase in lipid peroxidation/oxidative stress. PMID: 26369587
20. These results suggest that the low AChE activity observed in larynx squamous cell carcinoma may be useful for predicting the outcome of patients. PMID: 26002584
21. toxicogenetics/genetic association study in population in Turkey: Data suggest SNP in PON1 (192Q/R) is associated with susceptibility to organophosphate poisoning; plasma ACHE activities of exposed workers vary w/ PON1 genotype: 192RR>192QR>192QQ. PMID: 23625910
22. Data suggest cholinesterase inhibitors with high potency have proper conformation in active site of ACHE and interact with key residues (Trp84, Phe330 at catalytic anionic site; Trp279 at peripheral anionic site; Gly118, Gly119, Ala201 at oxyanion hole. PMID: 26202430
23. Phosphorylated p38, DNMT1 and AChE were aberrantly expressed in a subset of hepatocellular carcinoma tumors. PMID: 26299326
24. Report acetylcholinesterase kinetics using fluorogenic probe for the investigation of free thiols. PMID: 26494253
25. Report reactivation kinetics of a large series of bispyridinium oximes with organophosphate-inhibited human acetylcholinesterase. PMID: 26210933
26. Case Report: repetitive obidoxime treatment induced increase of red blood cell acetylcholinesterase activity even in a late phase of a severe methamidophos poisoning. PMID: 26200596
27. Low AChE activity in head and neck squamous cell carcinoma can be used to predict survival in patients with head and neck cancer. PMID: 25956553
28. Data show that the 3D-quantitative structure property relationship (QSAR) models are capable of explaining the experimental phenomenon of ligand recognition and binding to acetylcholinesterase (AChE). PMID: 24905476
29. The results suggest that interference with the enzymatic activities of AChE and/or interference with necroptosis may be novel approaches to influence ovarian functions. PMID: 25766324
30. PRX-105 is a plant-derived recombinant version of the human 'read-through' acetylcholinesterase splice variant (AChE-R) which may be used for treatment/prevention of organophosphate poisoning. PMID: 26051873
31. Data suggest that natural antisense RNA may play an important role in acetylcholinesterase (AChE) regulation via affecting the epigenetic modification in the AChE promoter region. PMID: 25240585
32. QSAR analysis on tacrine-related acetylcholinesterase inhibitors. PMID: 25239202
33. In symptomatic methylphosphonic difluoride poisoning high methylphosphonofluoridic acid concentrations in blood/tissues may lead to the formation of toxic phosphonyloximes after treatment with oximes. PMID: 25240274
34. The results showed that AChE clusters colocalized with neurexin assemblies in the neurites of hippocampal neurons. PMID: 24594013
35. Results suggest that AChE 7-20, a beta-hairpin region in AChE, might be a new motif in AChE capable of triggering Abeta aggregation and deposition PMID: 23981668
36. Synaptic acetylcholinesterase may be a tumor suppressor and is modulated by miR-212 in non-small cell lung cancer. PMID: 23974008
37. the T14 peptide derived from AChE produced a dose-dependent biphasic modulation of cortical networks activity dependent on the alpha-nAChR: study of a novel bioactive agent of high potential relevance to neurodegenerative disorders such as Alzheimer disease PMID: 23711548
38. The mesenteric lymphatic vessels show many AChE positive nerve fibres around their wall with an almost plexiform distribution. PMID: 24402754
39. The aim of this work is to review and discuss the recent findings about acetylcholinesterase, including its sensitivity to other pollutants and the expression of different splice variants. PMID: 23936791
40. There is decreased expression of ACHE and CHRM3 in eccrine glands of cholinergic urticaria patients. PMID: 23748235
41. AChE is regulated in two neuronal cell lines by APP in a manner independent of the generation of sAPPalpha, sAPPbeta, and AICD. PMID: 23897820
42. Low AChE activity was associated with deficits in neurodevelopment, particularly in attention, inhibition, and memory in boys but not in girls. PMID: 24249815
43. Data indicate that high AChE affinity of the compounds was achieved by optimizing different substituents on the pyridazinone ring, without sacrificing the AChE/BuChE selectivity profile. PMID: 23466605
44. verify amplification and/or deletion in the ACHE, BCHE, EPHB4 and MME genes in 32 samples of sporadic breast cancer PMID: 23063927
45. assayed the relative activities of AChE and BChE in membrane fractions and culture medium of three different neuronal cell lines, namely the neuroblastoma cell lines SH-SY5Y and NB7 and the basal forebrain cell line SN56 PMID: 23047022
46. AChE contains a deep active site gorge with two sites of ligand binding, an acylation site (or A-site) containing the catalytic triad at the base of the gorge and a peripheral site (or P-site) near the gorge entrance. PMID: 23047027
47. Free energy landscape for the binding process of Huperzine A to acetylcholinesterase PMID: 23440190
48. Acetylcholinesterase inhibitor huperazine A improved, or partly reversed, the Abeta-induced damage of neurite outgrowth. PMID: 23119107
49. AChE and BChE activities were decreased in prostate cancer patients PMID: 22560633
50. results suggest that the glycosylation may affect AChE(H) enzymatic activity and trafficking, but not dimer formation. The present findings indicate the significance of N-glycosylation in controlling the biosynthesis of the AChE(H) dimer form PMID: 22805525
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亞細胞定位：

Cell junction, synapse. Secreted. Cell membrane; Peripheral membrane protein.; [Isoform T]: Nucleus. Note=Only observed in apoptotic nuclei.; [Isoform H]: Cell membrane; Lipid-anchor, GPI-anchor; Extracellular side.
蛋白家族：

Type-B carboxylesterase/lipase family
組織特異性：

Isoform H is highly expressed in erythrocytes.
數(shù)據(jù)庫鏈接：

HGNC: 108

OMIM: 100740

KEGG: hsa:43

STRING: 9606.ENSP00000303211

UniGene: Hs.154495

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