Recombinant Escherichia coli Aquaporin Z (aqpZ) (Active)

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中文名稱：

重組大腸桿菌水蒸發蛋白Z（aqpZ）（活性）
品名簡稱：

Recombinant E.coli AQPZ protein (Active)
貨號：

CSB-CF352724ENV
說明書：

MSDS Datasheet
規格：

￥9720
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Activity
Measured by its binding ability in a functional ELISA. Immobilized aqpZ at 5 μg/ml can bind E.coli ytfE, the EC₅₀ of E.coli ytfE protein is 197.90-259.70 μg/ml.
其他：

產品詳情
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靶點詳情

產品詳情

純度：

Greater than 90% as determined by SDS-PAGE.
生物活性：

Measured by its binding ability in a functional ELISA. Immobilized aqpZ at 5 μg/ml can bind E.coli ytfE, the EC50 of E.coli ytfE protein is 197.90-259.70 μg/ml.
基因名：

aqpZ
Uniprot No.：

P60844
別名：

aqpZ; bniP; b0875; JW0859; Aquaporin Z; Bacterial nodulin-like intrinsic protein
種屬：

Escherichia coli (strain K12)
蛋白長度：

Full Length
來源：

in vitro E.coli expression system
分子量：

27.7kDa
表達區域：

1-231aa
氨基酸序列

MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFGLTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLIASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAGFAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGIIGGLIYRTLLEKRD
蛋白標簽：

N-terminal 6xHis-tagged
產品提供形式：

Liquid or Lyophilized powder
緩沖液：

Tris-based buffer，50% glycerol
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

3-7 business days
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
產品描述：

Recombinant Escherichia coli Aquaporin Z (aqpZ) (Active) is a full length protein expressed with N-terminal 6xHis-tagged in vitro E.coli expression system. Its expression region corresponds to 1-231aa of E.coli aqpZ protein. Its purity was determined by SDS-PAGE and reached up to 90% and presented a molecular mass band around 25 kDa on the gel. It was validated to be biologically active through functional ELISA analyses. This recombinant aqpZ protein may be used to synthesize antibodies against aqpZ or on the studies of aqpZ-associated signal transduction. This recombinant aqpZ proteins in-stock are available.
aqpZ is a channel that permits osmotically driven movement of water in both directions. It is involved in the osmoregulation and in the maintenance of cell turgor during volume expansion in rapidly growing cells. It mediates rapid entry or exit of water in response to abrupt changes in osmolarity. Evidence shows that induction of an immune response against AqpZ can trigger an autoimmune reaction against human Aqp4 and inflammation of the central nervous system.
Datasheet & COA：

Please contact us to get it.

產品評價

靶點詳情

功能：

Channel that permits osmotically driven movement of water in both directions. It is involved in the osmoregulation and in the maintenance of cell turgor during volume expansion in rapidly growing cells. It mediates rapid entry or exit of water in response to abrupt changes in osmolarity.
基因功能參考文獻：
1. Induction of an immune response against AqpZ or its homologous regions can also trigger an autoimmune reaction against human Aqp4 and inflammation of the central nervous system. PMID: 23008451
2. Data show that the open and closed states of AqpZ directly relate to the side chain conformation of residue R189. PMID: 21315687
3. the 2.5 A resolution structure of AqpZ suggests aquaporin selectivity results both from a steric mechanism due to pore size and from specific amino acid substitutions that regulate the preference for a hydrophobic or hydrophilic substrate PMID: 14691544
4. the difference in Arg-189 displacements is correlated with a strong electron density between first transmembrane helices of two open channels; the observed Arg-189 conformations are stabilized by asymmetrical subunit interactions in tAqpZ PMID: 16239219
5. analysis of of Escherichia coli aquaporin AqpZ and GlpF single-channel water permeabilities PMID: 16399837
6. biochemical analysis of permeability and solute transport characteristics of amphiphilic triblock-polymer vesicles containing the bacterial water-channel protein Aquaporin Z PMID: 18077364
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亞細胞定位：

Cell inner membrane; Multi-pass membrane protein.
蛋白家族：

MIP/aquaporin (TC 1.A.8) family
數據庫鏈接：

KEGG: ecj:JW0859

STRING: 316385.ECDH10B_0945