Whitlow linker Monoclonal Antibody
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中文名稱：Whitlow linker CAR檢測工具
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貨號(hào)：CSB-MA234468A0m
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規(guī)格：￥3000
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圖片：
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The Binding Activity of Whitlow linker with Anti-Whitlow linker antibody
Activity: Measured by its binding ability in a functional ELISA. Immobilized scFv-based Anti-CD19 CAR recombinant antibody at 2 μg/mL can bind Anti-Whitlow linker antibody. The EC50 is 5.147 to 5.761 ng/mL. -
Western Blot
Positive WB detected in: 293F whole cell lysate, scFv-based Anti-CD19 CAR containing a Whitlow linker (293F/CD19 CAR) whole cell lysate
All lanes: Whitlow linker antibody at 1:1000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 50-200 kDa
Observed band size: 50-200 KDa
Exposure time：5s -
Untransfected HEK-293F cells (green line) and transfected scFv-based Anti-CD19 CAR containing a Whitlow/218 linker stable cells (red line) were stained with anti-Whitlow linker antibody (2μg/1*106cells), washed and then followed by FITC-conjugated anti-Mouse IgG Fc antibody and analyzed with flow cytometry.
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其他：
產(chǎn)品詳情
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產(chǎn)品描述：CUSABIO貨號(hào)：CSB-MA234468A0m Whitlow linker單克隆抗體是針對(duì)人類Whitlow linker蛋白研發(fā)的高特異性科研試劑，適用于Western Blot、流式細(xì)胞術(shù)及ELISA等主流檢測平臺(tái)。該抗體經(jīng)多維度驗(yàn)證可特異性識(shí)別Whitlow linker靶點(diǎn)，該蛋白作為細(xì)胞連接復(fù)合體的重要組分，在維持組織屏障完整性和細(xì)胞間通訊中發(fā)揮關(guān)鍵作用。本品采用雜交瘤技術(shù)制備，具備嚴(yán)格的批次一致性，在蛋白質(zhì)印跡實(shí)驗(yàn)中能清晰呈現(xiàn)目標(biāo)條帶，流式檢測中可實(shí)現(xiàn)精準(zhǔn)的細(xì)胞表面標(biāo)記分析。研究人員可將其應(yīng)用于細(xì)胞黏附機(jī)制研究、上皮組織穩(wěn)態(tài)調(diào)控、細(xì)胞信號(hào)轉(zhuǎn)導(dǎo)通路解析等前沿領(lǐng)域，特別適用于探索Whitlow linker在細(xì)胞極性建立、創(chuàng)傷修復(fù)等生理過程中的分子機(jī)制。本產(chǎn)品為深入理解細(xì)胞連接相關(guān)蛋白網(wǎng)絡(luò)提供可靠工具，是開展細(xì)胞生物學(xué)、分子互作及疾病機(jī)理研究的優(yōu)選試劑，有效支持科研人員在蛋白質(zhì)功能研究與分子通路探索中獲得高質(zhì)量實(shí)驗(yàn)數(shù)據(jù)。
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基因名：Whitlow linker
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別名：Whitlow/218 Linker (E3U7Q); Whitlow linker
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宿主：Mouse
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反應(yīng)種屬：Homo sapiens (Human)
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免疫原：Whitlow linker-KLH
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標(biāo)記方式：Non-conjugated
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克隆類型：Monoclonal
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抗體亞型：IgG2b
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純化方式：>95%, Protein G purified
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克隆號(hào)：18A3G5
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濃度：It differs from different batches. Please contact us to confirm it.
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保存緩沖液：Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 -
產(chǎn)品提供形式：Liquid
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應(yīng)用范圍：ELISA, WB, FC
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推薦稀釋比：
Application Recommended Dilution WB WB：1:1000-1:5000 FC 1:50-1:200 -
Protocols：
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儲(chǔ)存條件：Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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貨期：Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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用途：For Research Use Only. Not for use in diagnostic or therapeutic procedures.
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