G4S linker Monoclonal Antibody

說明書

中文名稱：

G4S linker CAR檢測工具
貨號：

CSB-MA186062I2m
規格：

￥3000
圖片：
The Binding Activity of G4S linker with Anti-G4S linker antibody
Activity: Measured by its binding ability in a functional ELISA. Immobilized Three recombinant proteins containing G4S linker at 2 μg/mL can bind Anti-G4S linker antibody. The EC₅₀ is 10.03 to 17.44 ng/mL, 22.60 to 28.76 ng/mL and 6.478 to 10.27 ng/mL.

Untransfected HEK-293F cells (green line) and transfected scFv-based Anti-CD19 CAR containing a G4S linker stable cells (red line) were stained with anti-G4S linker antibody (2μg/1*10⁶cells), washed and then followed by APC-conjugated anti-Mouse IgG Fc antibody and analyzed with flow cytometry.
其他：

產品詳情

產品描述：

CUSABIO貨號：CSB-MA186062I2m是針對G4S linker靶點的單克隆抗體，適用于人源樣本的ELISA和流式細胞術檢測，推薦使用稀釋比例為1:50-1:200（FC）。該抗體特異性識別嵌合抗原受體（CAR）結構中連接輕鏈與重鏈的G4S柔性連接肽，作為CAR陽性率檢測的關鍵工具，可廣泛應用于CAR-T、CAR-NK等細胞療法的研發質控環節。在基礎研究中，該抗體能高效定位CAR胞外結構域，助力科研人員分析基因工程免疫細胞的表面表達效率、穩定性及功能驗證，為新型CAR細胞療法的開發提供精準檢測支持。產品采用雜交瘤技術制備，具有高批次穩定性和低交叉反應性，適用于細胞培養物、轉染樣本等實驗體系中的CAR分子定量分析，是腫瘤免疫治療機制研究與細胞工程技術開發的重要試劑。
基因名：

G4S linker
別名：

G4S, G4S linker
宿主：

Mouse
反應種屬：

Homo sapiens (Human)
免疫原：

G4S-BSA
標記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

IgG2b
純化方式：

>95%, Protein G purified
克隆號：

12F11C10
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
產品提供形式：

Liquid
應用范圍：

ELISA, FC

推薦稀釋比：

Application	Recommended Dilution
FC	1:50-1:200

Protocols：

ELISA Protocol
Flow cytometry (FC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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