Enterobacteria phage M13 III Monoclonal Antibody

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說明書

中文名稱：

大腸桿菌噬菌體M13 III鼠單克隆抗體
貨號：

CSB-MA303116A0m
規(guī)格：

￥1320
圖片：
Western Blot
Positive WB detected in: 1-3 lanes: Recombinant Enterobacteria phage M13 Attachment protein at 5ng, 10ng, 20ng; 4 lane: Enterobacteria phage M13 Attachment
All lanes: Enterobacteria phage M13 III antibody at 1:1000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 65, 44.652kDa
Observed band size: 65, 64kDa
Exposure time：3min
其他：

產(chǎn)品詳情
產(chǎn)品評價
相關(guān)產(chǎn)品
靶點詳情

產(chǎn)品詳情

產(chǎn)品描述：

Enterobacteria phage M13 III Monoclonal Antibody（CUSABIO貨號：CSB-MA303116A0m）是一款高特異性科研用抗體，靶向M13噬菌體的III型結(jié)構(gòu)蛋白。M13噬菌體作為絲狀噬菌體家族代表，其衣殼蛋白在基因工程領(lǐng)域具有重要價值，廣泛用于噬菌體展示技術(shù)構(gòu)建重組蛋白表達系統(tǒng)。該抗體采用雜交瘤技術(shù)制備，經(jīng)嚴(yán)格驗證可特異性識別Enterobacteria phage M13的III型蛋白表位，在ELISA實驗中展現(xiàn)高親和力結(jié)合特性，適用于Western Blot檢測重組噬菌體蛋白表達水平。產(chǎn)品經(jīng)過交叉反應(yīng)性測試，與大腸桿菌宿主蛋白無交叉反應(yīng)，保障實驗特異性，適用于噬菌體文庫篩選、重組蛋白表達驗證等分子生物學(xué)研究場景。作為噬菌體展示技術(shù)的關(guān)鍵檢測工具，本產(chǎn)品可高效支持抗體庫構(gòu)建、靶向肽篩選及病毒樣顆粒（VLP）研究，為基因工程、表位定位和蛋白質(zhì)相互作用研究提供可靠試劑。實驗方案已優(yōu)化適配常規(guī)檢測體系，滿足科研人員對M13噬菌體相關(guān)實驗的精準(zhǔn)檢測需求，是分子克隆與蛋白工程研究的理想選擇。
產(chǎn)品名稱：

Mouse anti-Enterobacteria phage M13 III Monoclonal antibody
Uniprot No.：

P69168
基因名：

III
別名：

III antibody; Attachment protein G3P antibody; Gene 3 protein antibody; G3P antibody; Minor coat protein antibody
宿主：

Mouse
反應(yīng)種屬：

Enterobacteria phage M13
免疫原：

Recombinant Enterobacteria phage M13 Attachment protein (19-424AA)
免疫原種屬：

Enterobacteria phage M13
標(biāo)記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

IgG1
純化方式：

>95%, Protein G purified
克隆號：

1B8E8
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
產(chǎn)品提供形式：

Liquid
應(yīng)用范圍：

ELISA, WB

推薦稀釋比：

Application	Recommended Dilution
WB	1:1000-1:5000

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評價

靶點詳情

功能：

Plays essential roles both in the penetration of the viral genome into the bacterial host via pilus retraction and in the extrusion process. During the initial step of infection, G3P mediates adsorption of the phage to its primary receptor, the tip of host F-pilus. Subsequent interaction with the host entry receptor tolA induces penetration of the viral DNA into the host cytoplasm. In the extrusion process, G3P mediates the release of the membrane-anchored virion from the cell via its C-terminal domain.
亞細胞定位：

Virion. Host membrane; Single-pass type I membrane protein. Note=Prior to assembly, G3P is found associated with the bacterial host inner membrane. There are about five copies of this protein per mature phage that are located on the head side of the filamentous virion.
蛋白家族：

Inovirus G3P protein family
數(shù)據(jù)庫鏈接：

KEGG: vg:927334

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