CD63 Monoclonal Antibody
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中文名稱：CD63鼠單克隆抗體
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貨號(hào)：CSB-MA004950A1m
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規(guī)格：￥1320
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圖片：
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Western Blot
Positive WB detected in: A549 whole cell lysate, Hela whole cell lysate, HepG2 whole cell lysate, MCF-7 whole cell lysate
All lanes CD63 antibody at 1:1000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 30-120 KD KDa
Observed band size: 30-120 KD KDa
Exposure time:1min -
Western Blot
Positive WB detected in: Raji whole cell lysate
All lanes CD63 antibody at 1:1000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 30-120 KD KDa
Observed band size: 30-120 KD KDa
Exposure time:1min -
Western Blot
Positive WB detected in: A375 whole cell lysate, Rabbit spleen tissue
All lanes CD63 antibody at 1:1000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 30-120 KD KDa
Observed band size: 30-120 KD KDa
Exposure time:1min` -
IHC image of CSB-MA004950A1m diluted at 1:500 and staining in paraffin-embedded human lung cancer tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at 37°C. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG labeled by HRP and visualized using 0.05% DAB.
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IHC image of CSB-MA004950A1m diluted at 1:500 and staining in paraffin-embedded human lung cancer tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at 37°C. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG labeled by HRP and visualized using 0.05% DAB.
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IHC image of CSB-MA004950A1m diluted at 1:500 and staining in paraffin-embedded human lung cancer tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at 37°C. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG labeled by HRP and visualized using 0.05% DAB.
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Immunofluorescence staining of A549 cells with CSB-MA004950A1m at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).
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Immunofluorescence staining of Hela cells with CSB-MA004950A1m at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).
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Immunofluorescence staining of MCF-7 cells with CSB-MA004950A1m at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).
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Overlay histogram showing A549 cells stained with CSB-MA004950A1m (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1μg/1*106cells) for 1 h at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG2b (1μg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
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Overlay histogram showing Hela cells stained with CSB-MA004950A1m (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1μg/1*106cells) for 1 h at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG2b (1μg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
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Overlay histogram showing HepG2 cells stained with CSB-MA004950A1m (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1μg/1*106cells) for 1 h at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG2b (1μg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
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Overlay histogram showing K562 cells stained with CSB-MA004950A1m (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1μg/1*106cells) for 1 h at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG2b (1μg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
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1. Exosomes extracted from Hela cells
2. Exosomes extracted from Hela cells
3. Exosomes extracted from urine
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其他：
產(chǎn)品詳情
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產(chǎn)品描述：CD63 Monoclonal Antibody（CUSABIO貨號(hào)：CSB-MA004950A1m）是專為科研實(shí)驗(yàn)設(shè)計(jì)的特異性檢測工具，靶向四次跨膜蛋白CD63分子。作為tetraspanin家族的重要成員，CD63廣泛參與細(xì)胞信號(hào)轉(zhuǎn)導(dǎo)、黏附分子互作及胞內(nèi)運(yùn)輸調(diào)控，尤其在腫瘤微環(huán)境動(dòng)態(tài)監(jiān)測、外泌體生物標(biāo)記物篩選及免疫細(xì)胞功能研究中具有重要科研價(jià)值。本產(chǎn)品采用高特異性單克隆抗體（克隆號(hào)2D8），經(jīng)嚴(yán)格驗(yàn)證適用于人、兔種屬樣本，可兼容ELISA、Western Blot、免疫組化、免疫熒光及流式細(xì)胞術(shù)五大實(shí)驗(yàn)平臺(tái)。其卓越的抗原識(shí)別能力為外泌體表面標(biāo)記物鑒定、溶酶體相關(guān)膜蛋白追蹤、免疫細(xì)胞激活狀態(tài)分析等前沿研究提供可靠保障，特別適用于腫瘤生物學(xué)、神經(jīng)退行性疾病機(jī)制探索及免疫調(diào)控相關(guān)課題。該抗體在多種樣本體系中展現(xiàn)優(yōu)異的批次穩(wěn)定性，是跨膜蛋白功能研究與細(xì)胞器動(dòng)態(tài)監(jiān)測實(shí)驗(yàn)的重要工具，廣泛服務(wù)于分子生物學(xué)實(shí)驗(yàn)室、生物醫(yī)學(xué)研究院所等科研場景，助力外泌體分離表征、腫瘤轉(zhuǎn)移機(jī)制解析等創(chuàng)新性研究。
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產(chǎn)品名稱：Mouse anti-Homo sapiens (Human) CD63 Monoclonal Antibody antibody
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Uniprot No.：
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基因名：CD63
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別名：CD63; MLA1; TSPAN30; CD63 antigen; Granulophysin; Lysosomal-associated membrane protein 3; LAMP-3; Lysosome integral membrane protein 1; Limp1; Melanoma-associated antigen ME491; OMA81H; Ocular melanoma-associated antigen; Tetraspanin-30; Tspan-30; CD antigen CD63
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宿主：Mouse
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反應(yīng)種屬：Human, Rabbit
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免疫原：Recombinant Human CD63 antigen protein (103-203AA)
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免疫原種屬：Homo sapiens (Human)
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標(biāo)記方式：Non-conjugated
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克隆類型：Monoclonal Antibody
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抗體亞型：IgG1
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純化方式：>95%, Protein G purified
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克隆號(hào)：10F11E6
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濃度：It differs from different batches. Please contact us to confirm it.
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保存緩沖液：Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 -
產(chǎn)品提供形式：Liquid
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應(yīng)用范圍：ELISA, WB, IHC, IF, FC
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推薦稀釋比：
Application Recommended Dilution WB WB：1:1000-1：8000 IHC 1:50-1:200 IF 1:50-1:200 FC 1:50-1:200 -
Protocols：
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儲(chǔ)存條件：Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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貨期：Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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用途：For Research Use Only. Not for use in diagnostic or therapeutic procedures.
引用文獻(xiàn)
產(chǎn)品評(píng)價(jià)
相關(guān)產(chǎn)品
靶點(diǎn)詳情
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功能：Functions as cell surface receptor for TIMP1 and plays a role in the activation of cellular signaling cascades. Plays a role in the activation of ITGB1 and integrin signaling, leading to the activation of AKT, FAK/PTK2 and MAP kinases. Promotes cell survival, reorganization of the actin cytoskeleton, cell adhesion, spreading and migration, via its role in the activation of AKT and FAK/PTK2. Plays a role in VEGFA signaling via its role in regulating the internalization of KDR/VEGFR2. Plays a role in intracellular vesicular transport processes, and is required for normal trafficking of the PMEL luminal domain that is essential for the development and maturation of melanocytes. Plays a role in the adhesion of leukocytes onto endothelial cells via its role in the regulation of SELP trafficking. May play a role in mast cell degranulation in response to Ms4a2/FceRI stimulation, but not in mast cell degranulation in response to other stimuli.
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基因功能參考文獻(xiàn)：
- Signaling focal contacts via CD63 were identified to facilitate cell adhesion, migration and mediate extracellular matrix-physical cues to modulate hematopoietic stem cells and progenitor cells function. PMID: 28566689
- Human CD63-GFP expression was controlled under the rat Sox2 promoter (Sox2/human CD63-GFP), and it was expressed in undifferentiated fetal brains. PMID: 29208635
- our results identify the CD63-syntenin-1-ALIX complex as a key regulatory component in post-endocytic HPV trafficking. PMID: 27578500
- CD63 and exosome expression is altered in scleroderma dermal fibroblasts PMID: 27443953
- CD63 is a critical player in LMP1 exosomal trafficking and LMP1-mediated enhancement of exosome production and may play further roles in limiting downstream LMP1 signaling. PMID: 27974566
- TIMP1 signaling via CD63 leads to activation of hepatic stellate cells, which create an environment in the liver that increases its susceptibility to pancreatic tumor cells. PMID: 27506299
- Concentrations of Cd63 were elevated in gingival crevicular fluid of patients with pre-eclampsia. PMID: 26988336
- CD163(+) TAMs in oral premalignant lesions coexpress CD163 and STAT1, suggesting that the TAMs in oral premalignant lesions possess an M1 phenotype in a Th1-dominated micromilieu. PMID: 26242181
- Our findings reveal that elevated levels of TIMP-1 impact on neutrophil homeostasis via signaling through CD63. PMID: 26001794
- These findings indicate that rhTIMP-1 promotes clonogenic expansion and survival in human progenitors via the activation of the CD63/PI3K/pAkt signaling pathway PMID: 26213230
- It was shown that treatment of macrophages with anti-CD63 inhibits CCR5-mediated virus infection in a cell type-specific manner, but that no inhibition of CXCR4-tropic viruses occurs. PMID: 25658293
- TM4SF5 interacted with CD151, and caused the internalization of CD63 from the cell surface. PMID: 25033048
- Diesel exhaust exposure during exercise induces platelet activation as illustrated by a dose-response increase in the release of CD62P and CD63. PMID: 25297946
- Data show that CD63 is a crucial player in the regulation of the tumor cell-intrinsic metastatic potential by affecting cell plasticity. PMID: 25354204
- CD63 tetraspanin is a negative driver of epithelial-to-mesenchymal transition in human melanoma cells. PMID: 24940653
- these results indicated that high glycosylation of CD63 by RPN2 is implicated in clinical outcomes in breast cancer patients PMID: 24884960
- Collectively, these findings indicate that CD63 may support Env-mediated fusion as well as a late (post-integration) step in the HIV-1 replication cycle. PMID: 24507450
- Data suggest that TIMP1 (tissue inhibitor of metalloproteinase-1) acts as chemoattractant for neural stem cells (NSC); TIMP1 enhances NSC adhesion, migration, and cytoskeletal reorganization; these effects are dependent on CD63/CD29 (integrin beta1). PMID: 24635319
- Timp1 is assembled in a supramolecular complex containing CD63 and beta1-integrins along melanoma genesis and confers anoikis resistance by activating PI3-K signaling pathway. PMID: 23522389
- Antigen-induced p38 MAPK phosphorylation in human basophils essentially contributes to CD63 upregulation PMID: 18727065
- Loss of CD63 has a similar phenotype to loss of P-selectin itself, thus CD63 is an essential cofactor to P-selectin. PMID: 21803846
- shRNA knockdown in B lymphoblastoid cell line results to increased CD4(+) T-cell recognition PMID: 21660937
- decreased levels of CD63 were associated with distant and lymph node metastasis status and does play a direct role in human gastric carcinogenesis. PMID: 21521534
- C-terminal modifications that retain LMP1 in Golgi compartments preclude assembly within CD63-enriched domains and/or exosomal discharge leading to NF-kappaB overstimulation. PMID: 21527913
- These findings suggest that CD63 plays an early post-entry role prior to or at the reverse transcription step. PMID: 21315401
- ameloblastin is expressed in osteoblasts and functions as a promoting factor for osteogenic differentiation via a novel pathway through the interaction between CD63 and integrin beta1 PMID: 21149578
- Surface expression of the novel CD63 variant is a distinguishing feature of mast cells, which are are stable, multiple-use cells capable of surviving and delivering several consecutive hits PMID: 20337613
- this work provides the first evidence of a TIMP-4/CD63 association in astrocytoma tumor cells PMID: 20693981
- CD63 expression results from only the anaphylactic degranulation form of histamine release. PMID: 20633031
- Serum sCD163 is a homogenous protein covering more than 94% of the CD163 ectodomain including the haptoglobin-hemoglobin -binding region. PMID: 19581020
- Results show that AP-3 is absolutely required for the delivery of CD63 to lysosomes via the trans-Golgi network. PMID: 11907283
- downregulation of CD63 antigen is associated with breast tumor progression PMID: 12579280
- possible role in HIV-1 infections specific for macrophages PMID: 12610138
- Post-translational modification of CD63 may be involved in the functional and morphological changes of MHC class II compartments that occur during dendritic cell maturation PMID: 12755696
- relationships between the expression levels of CD61, CD63, and PAC-1 on the platelet surface and the incidences of acute rejection and tubular necrosis as well as the recovery of graft function after renal transplantation PMID: 12826159
- Upon platelet interaction with fibrinogen, cholesterol accumulated at the tips of filopodia and at the leading edge of spreading cells; cholesterol-rich raft aggregation was accompanied by concentration of c-Src and CD63 in these cell domains PMID: 12871315
- The study on CD63 included its chemistry eg. if it had and O-linked carbohydrate that was digested with O-glycanase. PMID: 12974720
- CD63 serves as an adaptor protein that links its interaction partners to the endocytic machinery of the cell. PMID: 14660791
- results suggest that CD9, CD63, CD81, and CD82 could play a role in modulating the interactions between immature DCs and their environment, slowing their migratory ability. However, only CD63 would intervene in the internalization of complex antigens PMID: 15130945
- Constitutive expression of CD63 may indicate that this factor does not play a direct role in thyroid carcinogenesis PMID: 15375577
- CD63 represents an activation-induced reinforcing element, whose triggering promotes sustained and efficient T cell activation and expansion. PMID: 15528334
- The linkage of CD63 with PI 4-kinase may result in the recruitment of this signaling enzyme to specific membrane locations in the platelet where it influences phosphoinositide-dependent signaling and platelet spreading. PMID: 15711748
- This study identifies a trafficking pathway from CD63-positive multivesicular bodies to the bacterial inclusion, a novel interaction that provides essential lipids necessary for maintenance of a productive intracellular infection. PMID: 16410552
- CD63 is recruited to already-budded Weibel-Palade bodies by an AP-3-dependent route PMID: 16683915
- CD63-syntenin-1 complex is abundant on the plasma membrane PMID: 16908530
- CD63 is a cell surface binding partner for TIMP-1, regulating cell survival and polarization via TIMP-1 modulation of tetraspanin/integrin signaling complex. PMID: 16917503
- Chronic urticaria serum-induced CD63 expression assay performed on atopic whole blood by means of tricolor flow cytometry could be the most useful tool for identification of a subset of patients with autoimmune chronic urticaria. PMID: 16918509
- In conclusion, using well-defined experimental conditions, the measurement of CD203c up-regulation on basophils in response to specific allergens is as reliable as CD63-BAT for the in vitro diagnosis of patients with IgE-mediated allergy. PMID: 17275019
- positive correlation between CD63 and erythrocyte sedimentation rate in rheumatoid arthritis PMID: 17279322
- Results suggest that CD63 can be a biomarker for predicting the prognosis in early stages of lung adenocarcinoma. PMID: 17350713
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亞細(xì)胞定位：Cell membrane; Multi-pass membrane protein. Lysosome membrane; Multi-pass membrane protein. Late endosome membrane; Multi-pass membrane protein. Endosome, multivesicular body. Melanosome. Secreted, extracellular exosome. Cell surface.
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蛋白家族：Tetraspanin (TM4SF) family
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組織特異性：Detected in platelets (at protein level). Dysplastic nevi, radial growth phase primary melanomas, hematopoietic cells, tissue macrophages.
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