ALPP Monoclonal Antibody

說明書

中文名稱：

ALPP Monoclonal Antibody
貨號：

CSB-MA959669
規格：

￥1320
圖片：
Immunocytochemical staining of HepG2 cells with ALPP antibody. Nuclei were stained blue with DAPI; ALPP was stained magenta with Alexa Fluor? 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar: 20 μm.

Western blotting analysis using ALPP antibody. Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ALPP antibody and HRP-conjugated goat anti-mouse secondary antibody respectively.
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靶點詳情

產品詳情

產品名稱：

Mouse anti-Homo sapiens (Human) ALPP Monoclonal antibody
Uniprot No.：

P05187
基因名：

ALPP
別名：

ALPP; Alkaline Phosphatase, Placental; PLAP; PALP; Alkaline Phosphatase, Placental Type; Alkaline Phosphatase Regan Isozyme; Placental Alkaline Phosphatase 1; EC 3.1.3.1; PLAP-1; Alkaline Phosphatase, Placental (Regan Isozyme); Intestinal-Type Alkaline Phosphatase; Intestinal Alkaline Phosphatase; Placental Alkaline Phosphatase; Alkaline Phosphomonoesterase; Glycerophosphatase; Regan Isozyme; ALPI; ALP; IAP
宿主：

Mouse
反應種屬：

Human
免疫原：

Recombinant Human ALPP protein
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

Mouse IgG1
純化方式：

Affinity-chromatography
克隆號：

25G5
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
產品提供形式：

Liquid
應用范圍：

ELISA, WB, ICC

推薦稀釋比：

Application	Recommended Dilution
WB	1:2500-1:5000
ICC	1:100-1:1000

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Alkaline phosphatase that can hydrolyze various phosphate compounds.
基因功能參考文獻：
1. This meta-analysis suggests that high serum ALP level is obviously associated with lower OS rate in patients with osteosarcoma, and it is an effective biomarker of prognosis. PMID: 29970708
2. Concentrations of PLAP were elevated in gingival crevicular fluid of patients with pre-eclampsia. PMID: 26988336
3. SALL4 also outperformed PLAP on a small sample of cytology blocks. Although SALL4 is not entirely specific, it is a highly sensitive marker with strong diffuse nuclear reactivity in the majority of MGCTs in the posttreatment setting, at significantly higher levels than PLAP PMID: 25906119
4. Quantum-mechanical computational methods were employed to study the catalytic mechanism of human placental AP (PLAP). An active-site model was used, constructed on the basis of the X-ray crystal structure of the enzyme. PMID: 25409280
5. term placental explants, but not their conditioned medium, can de-phosphorylate IGFBP-1 through the action of placental alkaline phosphatase PMID: 24856042
6. Anti-PLAP antibodies may serve as a modular building blocks for the development of targeted therapeutic products, armed with cytotoxic drugs, radionuclides or cytokines as payloads. PMID: 24247025
7. A candidate gene, ALPP, encoding the placental alkaline phosphatase, was identified as being potentially involved in recurrent spontaneous abortion. PMID: 24296104
8. The objective of the following study was to record the specificity and sensitivity of alpha5(IV) loss, smoothelin expression and PLAP expression as markers of gastrointestinal smooth muscle neoplasms PMID: 24043717
9. Data indicate that p180 is required for the efficient targeting of placental alkaline phosphatase (ALPP) mRNA to the endoplasmic reticulum (ER). PMID: 24019514
10. Calculation of the electrostatic potentials within the active site of human placental alkaline phosphatase also suggests that the local positive electrostatic environment may account for its capability to distinguish various substrates PMID: 21910833
11. High serum alkaline phosphatase cooperating with MMP-9 is associated with metastasis in patients with primary osteosarcoma. PMID: 22333159
12. PLAP exerts a positive effect on DNA replication and acts as a proliferative factor in trophoblastic cells. PMID: 21868091
13. The proximity of undifferentiated gonadal tissue with the tumors as well as the immunostaining patterns (PLAP+, OCT3/4+, and CD117/KIT+) suggests that germ cells found in them are a risk factor for gonadal tumors. PMID: 21692598
14. the catalytic mechanism of human placental alkaline phosphatase PMID: 21939286
15. Data show that serum alkaline phosphatase, Gleason score, and intensity of bone metastasis are important and statistically significant prognostic factors, and affects time to progression and life time. PMID: 19450995
16. at a certain time point during adrenocortical development, some fetal zone cells survive owing to defective apoptosis and develop into childhood ACT, maintaining some characteristics of the embryonal period, such as PLAP expression PMID: 21516013
17. Serum total calcium (r -0.1362, p<0.001), serum inorganic phosphate (r -0.45, p<0.001) and serum alkaline phosphatase (r -0.5587, p<0.001) have shown inverse relationship with age. PMID: 20655896
18. High serum alkaline phosphatase is associated with chronic kidney disease. PMID: 20299338
19. differential expression of Pl(1) and Pl(2) probably results from linkage disequilibrium with the sequence variation rs2014683G>A in the ALPP gene promoter that was shown to have allele-specific binding patterns to placental nuclear proteins. PMID: 20663553
20. structure of placental alkaline phosphatase with pNPP contained only p-nitrophenol in three distinct sites, while the structure with 5'-AMP contained the p-nitrophenyl group in two of the sites instead of 5'-AMP PMID: 20693656
21. Low magnitudes of tensile strain enhance expression of alkaline phosphatase in human osteoblasts. PMID: 19595020
22. The PLAP D allele contains two amino acid substitutions: P209R (692C>G) and E429G (1352 A>G). PMID: 11857742
23. Proximity of the protein moiety of a GPI-anchored protein to the membrane surface: a FRET study. PMID: 12081485
24. the beta-N-acetylglucosaminyl phosphate diester residue is attached to the glycosylphosphatidylinositol anchor of human placental alkaline phosphatase and is a target of the channel-forming toxin aerolysin PMID: 12851398
25. receptor for Aeromonas sobria hemolysin. PMID: 15715171
26. analysis of human placental alkaline phosphatase in complex with functional ligands PMID: 15946677
27. The effect of parity on placental weight and birth weight is examined through a series of birth records from an Indian population in Calcutta. PMID: 16431676
28. crystal structure of strontium-substituted human placental alkaline phosphatase shows that strontium substitutes the calcium ion with concomitant modification of the metal coordination PMID: 16815919
29. activity of GPI-anchored enzymes may be modulated by membrane microenvironment features PMID: 18416535
30. Serum bilirubin, alkaline phosphatase, and aspartate aminotransferase are an efficient set of biochemistries to identify UDCA-treated patients with primary biliary cirrhosis at risk of death or liver transplantation (LT). PMID: 18752324
31. Elevations of alkaline phosphatase is associated with therapy related pediatric cancer. PMID: 18802949
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亞細胞定位：

Cell membrane; Lipid-anchor, GPI-anchor.
蛋白家族：

Alkaline phosphatase family
組織特異性：

Detected in placenta (at protein level).
數據庫鏈接：

HGNC: 439

OMIM: 171800

KEGG: hsa:250

STRING: 9606.ENSP00000375881

UniGene: Hs.284255

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