Sheep Tumor Necrosis Factor α,TNF-α ELISA Kit

Sheep Tumor Necrosis Factor α,TNF-α ELISA Kit

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中文名稱：

綿羊腫瘤壞死因子α(TNF-α)酶聯免疫試劑盒
貨號：

CSB-E13853Sh
規格：

96T/48T
價格：

￥4200/￥3000
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產品描述：

綿羊腫瘤壞死因子α(TNF-α)酶聯免疫試劑盒（CSB-E13853Sh）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的TNF含量。TNF（腫瘤壞死因子）是一種多功能促炎細胞因子，最初因能引起腫瘤組織壞死而得名。其超家族包括19個配體和29個受體，參與宿主防御、炎癥、細胞凋亡等生理過程。TNF通過與其受體結合，激活下游信號通路，調節細胞存活、凋亡和炎癥反應。試劑盒檢測范圍為3.125 pg/mL-50 pg/mL，TNF-α的動態監測對綿羊感染性疾病模型、疫苗研發及免疫機制研究具有重要價值；可高效支持動物醫學研究中炎癥相關疾病模型的建立、病原體感染后免疫應答評估以及抗炎藥物作用機制的體外實驗，為畜牧科研中的免疫調控研究提供可靠工具本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

TNF ELISA Kit; TNFA ELISA Kit; TNFSF2 ELISA Kit; Tumor necrosis factor ELISA Kit; Cachectin ELISA Kit; TNF-alpha ELISA Kit; Tumor necrosis factor ligand superfamily member 2 ELISA Kit; TNF-a) [Cleaved into: Tumor necrosis factor ELISA Kit; membrane form ELISA Kit; N-terminal fragment ELISA Kit; NTF); Intracellular domain 1 ELISA Kit; ICD1); Intracellular domain 2 ELISA Kit; ICD2); C-domain 1; C-domain 2; Tumor necrosis factor ELISA Kit; soluble form] ELISA Kit
縮寫：

TNF
Uniprot No.：

P23383
種屬：

Ovis aries (Sheep)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

3.125 pg/mL-50 pg/mL
靈敏度：

3.125 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Immunology
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of sheep TNF-α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	84
	Range %	80-88
1:2	Average %	95
	Range %	90-99
1:4	Average %	90
	Range %	85-94
1:8	Average %	102
	Range %	98-109

回收率：

The recovery of sheep TNF-α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	84-95
EDTA plasma (n=4)	96	90-101

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average
50	0.161	0.165	0.163
25	0.308	0.324	0.316
12.5	0.551	0.512	0.532
6.25	1.012	0.982	0.997
3.125	2.054	2.011	2.033

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

Physiological Responses, Oxidative Status and Productive Performance of Barki Sheep fed Dietary Addition with Quinoa Seeds and Exposed to Solar Radiation WA Fouda,/,2024
Impact of Vitamin D Supplement on Reproductive Performance in Pre-mating Ewes: Presumptive Regulatory Role of Some Inflammatory Cytokines and Serum Biomarkers GA El-Amrawi,Journal of Veterinary Sciences,2023
Immunological response of lame sheep to Clinical Interdigital Dermatitis and Footrot: Procalcitonin, Acute phase proteins, and Pro-inflammatory Cytokines W El-Deeb,Comparative immunology, microbiology and infectious diseases,2022
Characterization of immunological, biochemical and inflammatory response of clinical and subclinical endometritis in ewes in the subtropics N Nasreldin,Animal Reproduction Science,2020
Peripheral and placental immune responses in sheep after experimental infection with Toxoplasma gondii at the three terms of gestation Castano P, et al,Veterinary Research,2019
Effect of Central Injection of Neostigmine on the Bacterial Endotoxin Induced Suppression of GnRH/LH Secretion in Ewes during the Follicular Phase of the Estrous Cycle Herman A P, et al,International Journal of Molecular Sciences,2019
Acute phase proteins, proinflammatory cytokines and oxidative stress biomarkers in sheep, goats and she-camels with Coxiella burnetii infection-induced abortion El-Deeb W, et al,Comparative Immunology, Microbiology and Infectious Diseases,2019
Neostigmine Attenuates Proinflammatory Cytokine Expression in Preoptic Area but Not Choroid Plexus during Lipopolysaccharide-Induced Systemic Inflammation Andrzej P. Herman .et al,Mediators of Inflammation,2018
The diagnostic accuracy of acute phase proteins and proinflammatory cytokines in sheep with pneumonic pasteurellosis El-Deeb W M,.et al, PeerJ,2016
Lipoproteins profile, acute phase proteins, proinflammatory cytokines and oxidative stress biomarkers in sheep with pneumonic pasteurellosis El-Deeb W M.et al, Comparative Clinical Pathology,,2015
The proteome of Hypobaric Induced Hypoxic Lung: Insights from Temporal Proteomic Profiling for Biomarker Discovery Ahmad Y. et al,Sci Rep,2015

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功能：

Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Induces insulin resistance in adipocytes via inhibition of insulin-induced IRS1 tyrosine phosphorylation and insulin-induced glucose uptake. Induces GKAP42 protein degradation in adipocytes which is partially responsible for TNF-induced insulin resistance. Plays a role in angiogenesis by inducing VEGF production synergistically with IL1B and IL6.; The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells.
基因功能參考文獻：
1. The aim of the study was to assess the prognostic significance of Tumor Necrosis Factor-alpha, Interleukin-12(p40), Interleukin-6 and Interleukin 10 levels in cerebrospinal fluid (CSF) in sheep with encephalitic listeriosis. PMID: 25817420
2. The p38 MAPK inhibitor decreased (P < 0.01) gene expression of TNFa but its effect was not observed at the level of TNFa protein synthesis PMID: 24995301
3. TNF-alpha gene expression in multifidus was greater on both sides in the disc lesion animals than in the muscle of control animals. PMID: 24718080
4. Injection of peptidoglycan-polysaccharide resulted in fever, increased concentrations of TNF-alpha on the day of and the day after challenge, and decreased concentrations of progesterone on days 14 and 21. PMID: 20603027
5. These data are consistent with the effects of LPS on GH secretion originating through peripheral cytokine presentation to the pituitary, as well as a potential to act directly on selective populations of pituitary cells via CD14. PMID: 15899940
6. plasma IL-1 beta, TNF-alpha and MCP-1 may potentially serve as markers to monitor prognosis of pregnancy toxaemia in ewes PMID: 17221363
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亞細胞定位：

Cell membrane; Single-pass type II membrane protein.; [Tumor necrosis factor, membrane form]: Membrane; Single-pass type II membrane protein.; [Tumor necrosis factor, soluble form]: Secreted.; [C-domain 1]: Secreted.; [C-domain 2]: Secreted.
蛋白家族：

Tumor necrosis factor family
數據庫鏈接：

KEGG: oas:443540

UniGene: Oar.455

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.