Sheep Somatotropin(GH1) ELISA kit

Sheep Somatotropin(GH1) ELISA kit

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中文名稱：

綿羊生長激素(GH1)酶聯免疫試劑盒
貨號：

CSB-EL009407SH
規格：

96T/48T
價格：

￥4200/￥3000
促銷：
其他：

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產品描述：

綿羊生長激素(GH1)酶聯免疫試劑盒（CSB-EL009407SH）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的GH1含量。GH1 是生長激素 1 基因，其編碼生長激素。它在調節身體生長、細胞增殖與再生等方面扮演重要角色。研究機制主要聚焦于其信號通路，探究它如何與受體結合、激活下游信號，以調控生長發育相關基因表達，助力疾病治療研究。試劑盒檢測范圍為2.5 ng/mL-40 ng/mL，適用于科研領域探究生長激素在綿羊生理調控中的作用機制。本產品可支持畜牧科研中生長性能評估、營養代謝實驗、繁殖周期監測等研究場景，尤其適用于分析不同飼養條件或激素干預下GH1的動態變化。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

GH1 ELISA Kit; Somatotropin ELISA Kit; Growth hormone ELISA Kit
縮寫：

GH1
Uniprot No.：

P67930
種屬：

Ovis aries (Sheep)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

2.5 ng/mL-40 ng/mL
靈敏度：

0.675 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Signal Transduction
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of sheep GH1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	90-101
1:2	Average %	93
	Range %	87-98
1:4	Average %	96
	Range %	95-102
1:8	Average %	95
	Range %	89-99

回收率：

The recovery of sheep GH1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	90-102
EDTA plasma (n=4)	92	88-96

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average
40	0.173	0.175	0.174
20	0.308	0.312	0.310
10	0.639	0.644	0.642
5	1.401	1.408	1.405
2.5	2.261	2.265	2.263

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

Prepartum and Postpartum Feed Restrictions Affect Blood Metabolites and Hormones Reducing Colostrum and Milk Yields in Fat-Tailed Dairy Sheep M Zarri,Animals,2021

功能：

Plays an important role in growth control. Its major role in stimulating body growth is to stimulate the liver and other tissues to secrete IGF-1. It stimulates both the differentiation and proliferation of myoblasts. It also stimulates amino acid uptake and protein synthesis in muscle and other tissues.
基因功能參考文獻：
1. Association analyses showed that both g.498G>C and g.616G>A GH polymorphisms were significantly associated with several growth traits (at P < 0.01 or P < 0.05) in three investigated sheep breeds. PMID: 27706626
2. The effect leptin, orexin-A, and ghrelin on the secretion of luteinizing hormone (LH) and growth hormone by adenohypophyseal explants obtained from normally fed or fasted ewes during the breeding and nonbreeding seasons. PMID: 24906931
3. The results showed that the growth hormone genotypes are associated with estimated breeding values of growth traits. PMID: 21328104
4. The simultaneous increase of expression of hypothalamic NPY suggests NPY involvement in the regulation of psychoemotional stress through the somatotrophic axis in the female lambs. PMID: 20675289
5. These data are consistent with the effects of LPS on GH secretion originating through peripheral cytokine presentation to the pituitary, as well as a potential to act directly on selective populations of pituitary cells via CD14. PMID: 15899940
6. Results show that in comparison to the product of the ovine growth hormone (GH)2-N gene copy, the postulated product of the GH2-Z gene copy, the G9R/G63S mutein, has a higher affinity to the growth hormone receptor and elevated biological activity. PMID: 17850798
7. Results describe thet pulsatility of cerebrospinal fluid and plasma ghrelin levels in relation to growth hormone secretion and food intake in the sheep, and the effects of exogenous ghrelin on GH and feeding behavior. PMID: 18673413
8. study has demonstrated for the first time that ghrelin differentially modulates growth hormone secretory response to growth hormone-releasing hormone according to feeding states in ruminant animals PMID: 19342191
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亞細胞定位：

Secreted.
蛋白家族：

Somatotropin/prolactin family
數據庫鏈接：

KEGG: oas:443329

UniGene: Oar.641

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.