Sheep Interleukin-8,IL-8 ELISA Kit

文獻引用 (3) 說明書下載

中文名稱：

綿羊白介素8(IL-8/CXCL8)酶聯免疫試劑盒
貨號：

CSB-E13202Sh
規格：

96T/48T
價格：

￥3600/￥2500
促銷：
其他：

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綿羊白介素8(IL-8/CXCL8)酶聯免疫試劑盒（CSB-E13202Sh）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的CXCL8含量。CXCL8是重要的趨化因子，在炎癥和免疫反應中發揮關鍵作用。其背景在于可吸引中性粒細胞至炎癥部位。研究機制方面，主要是通過與相應受體結合，激活下游信號通路，影響細胞的遷移、增殖等行為，在感染、腫瘤等疾病研究中備受關注。試劑盒檢測范圍為6.25 pg/mL-400 pg/mL，本試劑盒適用于科研領域中對綿羊模型中炎癥相關信號通路的機制研究、藥物干預效果評估或疾病模型構建等實驗需求，96孔板規格設計滿足高通量樣本分析要求本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

CXCL8 ELISA Kit; IL8Interleukin-8 ELISA Kit; IL-8 ELISA Kit; C-X-C motif chemokine 8 ELISA Kit; Chemokine ELISA Kit; C-X-C motif) ligand 8 ELISA Kit
縮寫：

CXCL8
Uniprot No.：

P36925
種屬：

Ovis aries (Sheep)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

6.25 pg/mL-400 pg/mL
靈敏度：

1.56 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of sheep IL-8 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

Sample

Serum(n=4)

1:1

Average %

Range %

80-90

1:2

Average %

Range %

80-95

1:4

Average %

Range %

87-99

1:8

Average %

Range %

90-105

回收率：

The recovery of sheep IL-8 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	85-100
EDTA plasma (n=4)	96	91-103

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
400	2.312	2.231	2.272	2.094
200	1.753	1.854	1.804	1.626
100	1.289	1.339	1.314	1.136
50	0.824	0.887	0.856	0.678
25	0.558	0.597	0.578	0.400
12.5	0.492	0.472	0.482	0.304
6.25	0.308	0.297	0.303	0.125
0	0.184	0.171	0.178

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

引用文獻

Construction and Biological Characteristics of a Quadruple Gene-Deleted Strain of Orf Virus as a Vaccine Candidate J Zhang, R Xin, J Zhao, R Wu, D Su, M Li, Y Zhu,Viruses,2025
Characterization of immunological, biochemical and inflammatory response of clinical and subclinical endometritis in ewes in the subtropics N Nasreldin,Animal Reproduction Science,2020
Cerebral and lung effects of a new generation synthetic surfactant with SP┞ and SP┟ analogs in preterm lambs Carmen Rey-Santano PhD.et al,Pediatric Pulmonology,2017

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功能：

IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus.
基因功能參考文獻：
1. In response to C. abortus infection, both ovine and bovine turbinate cells produce CXCL8 mRNA and protein late in the bacterial developmental cycle. PMID: 26342452
2. IL-8 signaling does not mediate lipopolysaccharide-induced lung inflammation and lung maturation. PMID: 19574422
亞細胞定位：

Secreted.
蛋白家族：

Intercrine alpha (chemokine CxC) family
數據庫鏈接：

KEGG: oas:443418

UniGene: Oar.485

Sheep Interleukin-8,IL-8 ELISA Kit

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