Sheep Interleukin-8,IL-8 ELISA Kit
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中文名稱(chēng)：綿羊白介素8(IL-8/CXCL8)酶聯(lián)免疫試劑盒
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貨號(hào)：CSB-E13202Sh
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規(guī)格：96T/48T
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價(jià)格：￥3600/￥2500
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其他：
產(chǎn)品詳情
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產(chǎn)品描述：綿羊白介素8(IL-8/CXCL8)酶聯(lián)免疫試劑盒（CSB-E13202Sh）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的CXCL8含量。CXCL8是重要的趨化因子，在炎癥和免疫反應(yīng)中發(fā)揮關(guān)鍵作用。其背景在于可吸引中性粒細(xì)胞至炎癥部位。研究機(jī)制方面，主要是通過(guò)與相應(yīng)受體結(jié)合，激活下游信號(hào)通路，影響細(xì)胞的遷移、增殖等行為，在感染、腫瘤等疾病研究中備受關(guān)注。試劑盒檢測(cè)范圍為6.25 pg/mL-400 pg/mL，本試劑盒適用于科研領(lǐng)域中對(duì)綿羊模型中炎癥相關(guān)信號(hào)通路的機(jī)制研究、藥物干預(yù)效果評(píng)估或疾病模型構(gòu)建等實(shí)驗(yàn)需求，96孔板規(guī)格設(shè)計(jì)滿足高通量樣本分析要求本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
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別名：CXCL8 ELISA Kit; IL8Interleukin-8 ELISA Kit; IL-8 ELISA Kit; C-X-C motif chemokine 8 ELISA Kit; Chemokine ELISA Kit; C-X-C motif) ligand 8 ELISA Kit
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縮寫(xiě)：
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Uniprot No.：
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種屬：Ovis aries (Sheep)
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樣本類(lèi)型：serum, plasma, tissue homogenates
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檢測(cè)范圍：6.25 pg/mL-400 pg/mL
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靈敏度：1.56 pg/mL
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反應(yīng)時(shí)間：1-5h
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樣本體積：50-100ul
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檢測(cè)波長(zhǎng)：450 nm
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研究領(lǐng)域：Immunology
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測(cè)定原理：quantitative
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測(cè)定方法：Sandwich
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精密度：
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度：
To assess the linearity of the assay, samples were spiked with high concentrations of sheep IL-8 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 85 Range % 80-90 1:2 Average % 88 Range % 80-95 1:4 Average % 94 Range % 87-99 1:8 Average % 97 Range % 90-105 -
回收率：
The recovery of sheep IL-8 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 92 85-100 EDTA plasma (n=4) 96 91-103 -
標(biāo)準(zhǔn)曲線：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. 
pg/ml OD1 OD2 Average Corrected 400 2.312 2.231 2.272 2.094 200 1.753 1.854 1.804 1.626 100 1.289 1.339 1.314 1.136 50 0.824 0.887 0.856 0.678 25 0.558 0.597 0.578 0.400 12.5 0.492 0.472 0.482 0.304 6.25 0.308 0.297 0.303 0.125 0 0.184 0.171 0.178 -
數(shù)據(jù)處理：
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貨期：3-5 working days
引用文獻(xiàn)
- Characterization of immunological, biochemical and inflammatory response of clinical and subclinical endometritis in ewes in the subtropics N Nasreldin,Animal Reproduction Science,2020
- Cerebral and lung effects of a new generation synthetic surfactant with SP┞ and SP┟ analogs in preterm lambs Carmen Rey-Santano PhD.et al,Pediatric Pulmonology,2017
產(chǎn)品評(píng)價(jià)
相關(guān)產(chǎn)品
靶點(diǎn)詳情
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功能：IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus.
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基因功能參考文獻(xiàn)：
- In response to C. abortus infection, both ovine and bovine turbinate cells produce CXCL8 mRNA and protein late in the bacterial developmental cycle. PMID: 26342452
- IL-8 signaling does not mediate lipopolysaccharide-induced lung inflammation and lung maturation. PMID: 19574422
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亞細(xì)胞定位：Secreted.
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蛋白家族：Intercrine alpha (chemokine CxC) family
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數(shù)據(jù)庫(kù)鏈接：
KEGG: oas:443418
UniGene: Oar.485
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