Sheep Interleukin 1β,IL-1β ELISA Kit

Sheep Interleukin 1β,IL-1β ELISA Kit

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文獻引用 (10) 說明書下載

中文名稱：

綿羊白介素1β(IL-1β)酶聯(lián)免疫試劑盒
貨號：

CSB-E10115Sh
規(guī)格：

96T/48T
價格：

￥3800/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產(chǎn)品描述：

綿羊白介素1β(IL-1β)酶聯(lián)免疫試劑盒（CSB-E10115Sh）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的IL1B含量。IL1B，即白細胞介素-1β，是一種重要的促炎細胞因子，參與先天免疫反應(yīng)。它由免疫和非免疫細胞在炎癥信號刺激下產(chǎn)生，通過炎癥小體激活和蛋白水解加工，與多種細胞因子協(xié)同作用，調(diào)節(jié)炎癥和免疫反應(yīng)，對腫瘤的發(fā)生、發(fā)展和轉(zhuǎn)移具有重要作用。試劑盒檢測范圍為15.6 pg/mL-1000 pg/mL，科研人員可借助定量分析感染模型、自身免疫疾病研究或藥物干預(yù)實驗中不同生物樣本的IL - 1β濃度變化，為炎癥機制探索、免疫調(diào)控研究及生物制劑開發(fā)提供可靠數(shù)據(jù)支持。所有檢測結(jié)果僅用于科學研究目的。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

IL1BInterleukin-1 beta ELISA Kit; IL-1 beta ELISA Kit
縮寫：

IL1B
Uniprot No.：

P21621
種屬：

Ovis aries (Sheep)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

15.6 pg/mL-1000 pg/mL
靈敏度：

3.9 pg/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of sheep IL-1β in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	86
	Range %	83-90
1:2	Average %	98
	Range %	92-102
1:4	Average %	100
	Range %	96-104
1:8	Average %	94
	Range %	90-96

回收率：

The recovery of sheep IL-1β spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	90-97
EDTA plasma (n=4)	97	94-99

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1000	2.487	2.456	2.472	2.371
500	2.031	2.000	2.016	1.915
250	1.614	1.568	1.591	1.490
125	1.105	1.023	1.064	0.963
62.5	0.703	0.689	0.696	0.595
31.2	0.421	0.402	0.412	0.311
15.6	0.275	0.262	0.269	0.168
0	0.103	0.099	0.101

數(shù)據(jù)處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

Beta-Glucans Improve the Mammary Innate Immune Response to Endotoxin Challenge in Dairy Ewes SA Guamán, A Elhadi, AAK Salama,Animals: an Open Access Journal from MDPI,2024
Immunological response of lame sheep to Clinical Interdigital Dermatitis and Footrot: Procalcitonin, Acute phase proteins, and Pro-inflammatory Cytokines W El-Deeb,Comparative immunology, microbiology and infectious diseases,2022
Direct administration of the non-competitive interleukin-1 receptor antagonist rytvela transiently reduced intrauterine inflammation in an extremely preterm sheep model of chorioamnionitis Yuki Takahashi,PLoS One,2021
Effect of Central Injection of Neostigmine on the Bacterial Endotoxin Induced Suppression of GnRH/LH Secretion in Ewes during the Follicular Phase of the Estrous Cycle Herman A P, et al,International Journal of Molecular Sciences,2019
Acute phase proteins, proinflammatory cytokines and oxidative stress biomarkers in sheep, goats and she-camels with Coxiella burnetii infection-induced abortion El-Deeb W, et al,Comparative Immunology, Microbiology and Infectious Diseases,2019
Neostigmine Attenuates Proinflammatory Cytokine Expression in Preoptic Area but Not Choroid Plexus during Lipopolysaccharide-Induced Systemic Inflammation Andrzej P. Herman .et al,Mediators of Inflammation,2018
The diagnostic accuracy of acute phase proteins and proinflammatory cytokines in sheep with pneumonic pasteurellosis El-Deeb W M,.et al,PeerJ,2016
Lipoproteins profile, acute phase proteins, proinflammatory cytokines and oxidative stress biomarkers in sheep with pneumonic pasteurellosis El-Deeb W M.et al, Comparative Clinical Pathology,,2015
Induction of Interleukin-8 and Interleukin-12 in Neonatal Ovine Lung Following Experimental Inoculation of Bovine Respiratory Syncytial Virus E. Redondo et al,Journal of Comparative Pathology,2014
Serum quantitative proteomic analysis reveals potential zinc-associated biomarkers for nonbacterial prostatitis. Yang X. et al,Prostate,2015

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功能：

Potent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells. Plays a role in angiogenesis by inducing VEGF production synergistically with TNF and IL6.
基因功能參考文獻：
1. Intravenous injection of SB203580 successfully inhibited synthesis of IL-1beta and reduced the production of IL-6 in the hypothalamus. PMID: 24995301
2. Interleukin-1beta was increased only on the injured side of multifidus in disc disease. PMID: 24718080
3. Inactivation of RhoA due to lack of prenylation leads to Rac1 activation and subsequent priming for IL-1beta secretion. PMID: 25107911
4. RANTES-403 was significantly associated with the risk of developing diffuse-type gastric cancer in men and found a possible gene-gene interaction between RANTES and IL-1B polymorphisms in gastric cancer carcinogenesis. PMID: 24323564
5. In vitro studies with IL-1-beta and TNF-alpha challenges, both proinflammatory cytokines resulted in elevated levels of CCL5 in conditioned media (p<0.01) PMID: 24468005
6. These results showed that IL-1beta and IL-1 receptor types I and II were expressed in the ovine hypothalamus in response to lipopolysaccharide injection. PMID: 20345592
7. These data are consistent with the effects of LPS on GH secretion originating through peripheral cytokine presentation to the pituitary, as well as a potential to act directly on selective populations of pituitary cells via CD14. PMID: 15899940
8. plasma IL-1 beta, TNF-alpha and MCP-1 may potentially serve as markers to monitor prognosis of pregnancy toxaemia in ewes PMID: 17221363
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亞細胞定位：

Cytoplasm, cytosol. Lysosome. Secreted, extracellular exosome. Secreted.
蛋白家族：

IL-1 family
數(shù)據(jù)庫鏈接：

KEGG: oas:443539

UniGene: Oar.434

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.