Rat von Willebrand Factor,vWF ELISA Kit

Rat von Willebrand Factor,vWF ELISA Kit

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中文名稱：

大鼠血管性血友病因子/瑞斯托霉素輔因子(VWF)酶聯免疫試劑盒
貨號：

CSB-E08438r
規格：

96T/48T
價格：

￥3600/￥2500
促銷：
其他：

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產品描述：

大鼠血管性血友病因子/瑞斯托霉素輔因子(VWF)酶聯免疫試劑盒（CSB-E08438r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的VWF含量。VWF即血管性血友病因子，在止血過程中發揮重要作用。其背景上與血管性血友病等疾病密切相關。研究機制方面，它能介導血小板黏附于受損血管壁，還可與凝血因子Ⅷ結合，保護其不被快速降解，目前圍繞它的研究有助于疾病診斷與治療。試劑盒檢測范圍為0.312 ng/mL-20 ng/mL，適用于心血管疾病模型構建、血栓機制研究、藥物療效評估等科研場景。為探索VWF在生理病理過程中的作用提供高效工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

Vwf ELISA Kit; von Willebrand factor ELISA Kit; vWF ELISA Kit; Fragment ELISA Kit
縮寫：

VWF
Uniprot No.：

Q62935
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

0.312 ng/mL-20 ng/mL
靈敏度：

0.078 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Cardiovascular
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat vWF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	91
	Range %	86-99
1:200	Average %	97
	Range %	92-105
1:400	Average %	85
	Range %	81-87
1:800	Average %	88
	Range %	83-95

回收率：

The recovery of rat vWF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	87-93
EDTA plasma (n=4)	95	90-100

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
20	2.300	2.488	2.394	2.257
10	2.011	2.102	2.057	1.920
5	1.446	1.560	1.503	1.366
2.5	0.903	0.900	0.902	0.765
1.25	0.628	0.586	0.607	0.470
0.625	0.350	0.364	0.357	0.220
0.312	0.242	0.258	0.250	0.113
0	0.134	0.140	0.137

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

The Role of Neuregulin-1 in Steatotic and Non-Steatotic Liver Transplantation from Brain-Dead Donors M Micó-Carnero,Biomedicines,2022
Unique Novel role of Adropin in the gastric Ulcer in a rotenone-induced Rat model of Parkinson's disease RE Abo El gheit,ACS Chemical Neuroscience,2020
Paeonol protects against hypertension in spontaneously hypertensive rats by restoring vascular endothelium Gai Z, et al,Bioscience Biotechnology and Biochemistry,2019
The liver sinusoidal endothelial cell damage in rats caused by heatstroke Xingqin Zhang.et al,European Journal of Inflammation,2018
The enhancement of serotonin-induced contraction of rat femoral artery is mediated by angiotensin II release from intact endothelium Marko Stojanovi?.et al,Archives of physiology and biochemistry ,2018
Hypolipidemic and anti-inflammatory effects of aorta and heart tissues of cattle and pigs in the atherosclerosis rat model Chernukha IM.et al,Anim Sci J,2018
Impaired NO-mediated vasodilatation in rat coronary arteries after asphyxial cardiac arrest. Troelsen TT.et al,Acta Anaesthesiol Scand.,2015
Endosulfan inducing blood hypercoagulability and endothelial cells apoptosis via the death receptor pathway in Wistar rats Wei J L.et al,Toxicology Research,,2015
Impaired NO-mediated vasodilatation in rat coronary arteries after asphyxial cardiac arrest T. T. TROELSEN et al,Anesthesia & Pain Management,2015
Mechanism of action of antiplatelet drugs on decompression sickness in rats: a protective effect of anti-GPIIbIIIa therapy Lambrechts K. et al,J Appl Physiol,2015
Vascular Endothelial Cell Injury Partly Induced by Mesenteric Lymph in Heat Stroke Tong H et al,Inflammation,2013
Role of Activation of 5´-Adenosine Monophosphate-Activated Protein Kinase in Gastric Ulcer Healing in Diabetic Rats Baraka A.M. et al,Pharmacology,2011
Role of modulation of vascular endothelial growth factor and tumor necrosis factor-alpha in gastric ulcer healing in diabetic rats Azza M. Baraka et al,biochemical pharmacology,2010

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功能：

Important in the maintenance of hemostasis, it promotes adhesion of platelets to the sites of vascular injury by forming a molecular bridge between sub-endothelial collagen matrix and platelet-surface receptor complex GPIb-IX-V. Also acts as a chaperone for coagulation factor VIII, delivering it to the site of injury, stabilizing its heterodimeric structure and protecting it from premature clearance from plasma.
亞細胞定位：

Secreted. Secreted, extracellular space, extracellular matrix.
組織特異性：

Plasma.
數據庫鏈接：

STRING: 10116.ENSRNOP00000026643

UniGene: Rn.35561

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.