Rat thrombomodulin,TM ELISA Kit

產(chǎn)品詳情

文獻(xiàn)引用 (4) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

大鼠血栓調(diào)節(jié)蛋白(TM)酶聯(lián)免疫試劑盒（CSB-E07939r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、細(xì)胞裂解物樣本中的TM含量。大鼠血栓調(diào)節(jié)蛋白（TM）是一種由內(nèi)皮細(xì)胞表達(dá)的凝血酶結(jié)合蛋白，具有抗凝、抑制血小板聚集和纖溶等作用。其表達(dá)與深靜脈血栓形成（DVT）的病理過程密切相關(guān)，可能成為DVT早期診斷和治療的潛在靶點(diǎn)。試劑盒檢測范圍為1.56 ng/mL-100 ng/mL，科研人員可將其應(yīng)用于心血管疾病模型、藥物作用機(jī)制、內(nèi)皮細(xì)胞功能研究等領(lǐng)域，例如評(píng)估病理狀態(tài)下TM的表達(dá)變化或探究干預(yù)措施對(duì)凝血調(diào)控的影響，為體外實(shí)驗(yàn)提供可靠的定量分析工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

N/A
縮寫：

TM
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, tissue homogenates, cell lysates
檢測范圍：

1.56 ng/mL-100 ng/mL
靈敏度：

0.39 ng/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Cardiovascular
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat TM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	99
	Range %	87-95
1:2	Average %	94
	Range %	89-97
1:4	Average %	99
	Range %	94-102
1:8	Average %	95
	Range %	91-99

回收率：

The recovery of rat TM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	91-97
EDTA plasma (n=4)	95	92-97

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.