Pig thrombomodulin,TM ELISA Kit

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產品詳情

產品描述：

豬血栓調節蛋白(TM)酶聯免疫試劑盒（CSB-E06530p）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的TM含量。豬血栓調節蛋白（TM）是一種重要的凝血調節因子，其通過與凝血酶結合，激活蛋白C，達到抗凝和促纖溶作用。基因工程豬腎臟移植中的分子檢測顯示，TM的表達有助于防止血栓形成和出血并發癥，是異種移植中重要的免疫調節和抗凝因子。試劑盒檢測范圍為0.312 ng/mL-20 ng/mL，適用于實驗室研究豬心血管疾病模型、凝血功能異常機制、炎癥相關病理過程及內皮細胞功能評估等領域本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

N/A
縮寫：

TM
種屬：

Sus scrofa (Pig)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

0.312 ng/mL-20 ng/mL
靈敏度：

0.078 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Cardiovascular
測定原理：

quantitative
測定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig TM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	91
	Range %	86-95
1:2	Average %	97
	Range %	92-103
1:4	Average %	85
	Range %	81-87
1:8	Average %	89
	Range %	83-96

回收率：

The recovery of pig TM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	87-96
EDTA plasma (n=4)	110	103-118

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.