Rat platelet activating factor,PAF ELISA KIT

產品詳情

文獻引用 (2) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

大鼠血小板活化因子(PAF)酶聯免疫試劑盒（CSB-E07930r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的PAF含量。血小板活化因子（PAF）是一種具有廣泛生物活性的磷脂介質。其在炎癥、過敏、心血管疾病等病理過程中扮演重要角色。研究機制集中在PAF與其受體結合，激活細胞內信號通路，引發一系列生理效應，是藥物研發的潛在重要靶點。試劑盒檢測范圍為1.56 ng/mL-100 ng/mL，適用于科研場景下對大鼠模型中PAF表達的動態分析，例如評估炎癥性疾病動物模型的PAF釋放水平、研究藥物或基因干預對PAF信號通路的影響，或探索PAF在心血管、免疫相關疾病中的作用機制本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

N/A
縮寫：

PAF
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

1.56 ng/mL-100 ng/mL
靈敏度：

0.39 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Biochemicals
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat PAF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	98
	Range %	92-104
1:2	Average %	92
	Range %	87-98
1:4	Average %	89
	Range %	86-98
1:8	Average %	104
	Range %	96-108

回收率：

The recovery of rat PAF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	85	80-92
EDTA plasma (n=4)	92	88-96

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.