Human platelet activating factor,PAF ELISA Kit
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中文名稱：
人血小板活化因子(PAF)酶聯免疫試劑盒
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貨號：
CSB-E07929h
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規格：
96T/48T
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價格：
￥3200/￥2500
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其他：
產品詳情
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產品描述：
人血小板活化因子(PAF)酶聯免疫試劑盒（CSB-E07929h）為雙抗夾心法ELISA試劑盒，定量檢測血清、尿液、組織培養上清液、組織勻漿、細胞裂解物樣本中的PAF含量。PAF即血小板活化因子，廣泛參與炎癥反應、過敏反應等。其背景在于它是一種強效的內源性磷脂介質。研究機制上，主要通過與特定受體結合，激活細胞內信號通路，引發系列生理病理反應，在多種疾病發生發展中扮演重要角色。試劑盒檢測范圍為4.69 ng/mL-300 ng/mL，該產品可為心血管疾病機制研究、炎癥反應模型構建、藥物靶點篩選等科研領域提供PAF濃度的精準檢測，尤其適用于體外實驗模型中PAF動態變化的定量分析，幫助研究者深入探究其在細胞信號傳導、免疫調控及病理過程中的作用機制。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
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別名：
N/A
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縮寫：
PAF
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種屬：
Homo sapiens (Human)
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樣本類型：
serum, urine, cell culture supernates, tissue homogenates, cell lysates
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檢測范圍：
4.69 ng/mL-300 ng/mL
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靈敏度：
1.17 ng/mL
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反應時間：
1-5h
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樣本體積：
50-100ul
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檢測波長：
450 nm
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研究領域：
Biochemicals
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測定原理：
quantitative
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測定方法：
Sandwich
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精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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線性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human PAF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 85 | |
| Range % | 81-92 | |
| 1:2 | Average % | 95 | |
| Range % | 90-105 | |
| 1:4 | Average % | 94 | |
| Range % | 91-102 | |
| 1:8 | Average % | 90 | |
| Range % | 80-98 | |
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回收率：
| The recovery of human PAF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 91 | 88-100 | |
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標準曲線：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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 | ng/ml | OD1 | OD2 | Average | Corrected | | | 300 | 2.097 | 2.093 | 2.095 | 1.994 | | | 150 | 1.823 | 1.793 | 1.808 | 1.707 | | | 75 | 1.476 | 1.374 | 1.425 | 1.324 | | | 37.5 | 1.029 | 0.986 | 1.008 | 0.907 | | | 18.75 | 0.782 | 0.772 | 0.777 | 0.676 | | | 9.38 | 0.421 | 0.401 | 0.411 | 0.310 | | | 4.69 | 0.218 | 0.202 | 0.210 | 0.109 | | | 0 | 0.105 | 0.097 | 0.101 | | |
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本試劑盒所含材料：
- A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human PAF antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
- Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
- One vial Biotin-labeled PAF antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
- One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
- One vial Biotin-conjugateDiluent (15 ml/bottle) ---Dilute the Biotin-antibody.
- One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
- One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
- One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
- One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
- One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
- Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
- An instruction manual
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本試劑盒不含材料：
- A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
- An incubator can provide stable incubation conditions up to 37°C±5°C.
- Centrifuge
- Vortex
- Squirt bottle, manifold dispenser, or automated microplate washer
- Absorbent paper for blotting the microtiter plate
- 50-300ul multi-channel micropipette
- Pipette tips
- Single-channel micropipette with different ranges
- 100ml and 500ml graduated cylinders
- Deionized or distilled water
- Timer
- Test tubes for dilution
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數據處理：
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貨期：
3-5 working days
引用文獻
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Assessment of Some Platelet Activating Markers and Secretory Status with Clinical Manifestations in Multiple Sclerosis Iraqi Patients
KM Salih,Al-Mustansiriyah Journal of Science,2022
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Ursodeoxycholic acid protects neonatal rats from necrotizing enterocolitis: a biochemical, histopathological, and immunohistochemical study
E S?nmezg?z,The Journal of Maternal-Fetal & Neonatal Medicine,2020
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Hypoxic-ischemic enterocolitis: a proposal of a new terminology for early NEC or NEC-like disease in preterm infants, a single-center prospective observational study
Onay O S, et al,European Journal of Pediatrics,2019
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Altered monocyte response to the dengue virus in those with varying severity of past dengue infection
Kamaladasa A, et al,Antiviral Research,2019
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Secretory phospholipase A2 in the pathogenesis of acute dengue infection
Chandima Jeewandara.et al,Immunity, Inflammation and Disease,2016
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Platelet activating factor contributes to vascular leak in acute dengue infection
Jeewandara C et al,PLoS Negl Trop Dis,2015
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The association of MDR1 C3435T and G2677T/A polymorphisms with plasma platelet-activating factor levels and coronary artery disease risk in Turkish population
Ayaz G et al,Gene,2013
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Characterization of antioxidant/anti-inflammatory properties and apoA-I-containing subpopulations of HDL from family subjects with monogenic low HDL disorders
Masakazu Kurita et al,Clinica Chimica Acta,2011
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