Rat placenta growth factor,PLGF ELISA kit
產品詳情
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產品描述：
大鼠胎盤生長因子(PLGF)酶聯(lián)免疫試劑盒（CSB-E07400r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的PGF含量。PGF（胎盤生長因子）是VEGF家族成員之一，主要在胎盤滋養(yǎng)層細胞中表達，對血管生成和胚胎滋養(yǎng)層生長至關重要。其研究機制涉及通過結合VEGF受體促進血管內皮細胞增殖和遷移，增強血管通透性與穩(wěn)定性。PGF在多種病理過程中發(fā)揮作用，如心血管疾病和腫瘤生長，具有廣泛的生物學意義和研究價值。試劑盒檢測范圍為3.12 pg/mL-200 pg/mL，支持科研人員開展大鼠模型中妊娠并發(fā)癥機制、組織修復調控或血管生成相關疾病研究。兼容多種生物樣本類型，特別適用于探究胎盤發(fā)育異常、心血管疾病模型或腫瘤微環(huán)境中的PLGF動態(tài)變化，為體外實驗提供可靠的定量分析工具。本品僅用于科研，不用于臨床診斷，產品具體參數(shù)及操作步驟詳見產品說明書。
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別名：
Pgf ELISA Kit; Plgf ELISA Kit; Placenta growth factor ELISA Kit; PlGF ELISA Kit
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縮寫：
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Uniprot No.：
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種屬：
Rattus norvegicus (Rat)
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樣本類型：
serum, plasma, tissue homogenates
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檢測范圍：
3.12 pg/mL-200 pg/mL
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靈敏度：
0.78 pg/mL
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反應時間：
1-5h
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樣本體積：
50-100ul
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檢測波長：
450 nm
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研究領域：
Cancer
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測定原理：
quantitative
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測定方法：
Sandwich
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精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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線性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat PLGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
92 |
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| Range % |
88-95 |
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| 1:2 |
Average % |
94 |
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| Range % |
89-98 |
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| 1:4 |
Average % |
93 |
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| Range % |
87-97 |
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| 1:8 |
Average % |
99 |
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| Range % |
93-102 |
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回收率：
| The recovery of rat PLGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
93 |
89-97 |
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| EDTA plasma (n=4) |
94 |
91-98 |
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標準曲線：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 200 |
2.246 |
2.234 |
2.240 |
2.085 |
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| 100 |
1.472 |
1.456 |
1.464 |
1.309 |
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| 50 |
0.901 |
0.914 |
0.908 |
0.753 |
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| 25 |
0.639 |
0.649 |
0.644 |
0.489 |
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| 12.5 |
0.364 |
0.389 |
0.377 |
0.222 |
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| 6.25 |
0.300 |
0.308 |
0.304 |
0.149 |
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| 3.12 |
0.252 |
0.242 |
0.247 |
0.092 |
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| 0 |
0.156 |
0.154 |
0.155 |
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本試劑盒所含材料：
- A micro ELISA plate --The 96-well plate has been pre-coated with an anti-rat PLGF antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
- Two vials lyophilized standard --Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
- One vial Biotin-labeled PLGF antibody (100 x concentrate) (120 μl/bottle) --Act as the detection antibody.
- One vial HRP-avidin (100 x concentrate) (120 μl/bottle) --Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
- One vial Biotin-antibody Diluent (15 ml/bottle) --Dilute the high concentration Biotin-antibody to an appropriate working solution.
- One vial HRP-avidin Diluent (15 ml/bottle) --Dilute the high concentration HRP-avidin solution to an appropriate solution.
- One vial Sample Diluent (50 ml/bottle)--Dilute the sample to an appropriate concentration.
- One vial Wash Buffer (25 x concentrate) (20 ml/bottle) --- Wash away unbound or free substances.
- One vial TMB Substrate (10 ml/bottle) --Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
- One vial Stop Solution (10 ml/bottle) --Stop the color reaction. The solution color immediately turns from blue to yellow.
- Four Adhesive Strips (For 96 wells) --Cover the microplate when incubation.
- An instruction manual
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本試劑盒不含材料：
- A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
- An incubator can provide stable incubation conditions up to 37°C±5°C.
- Centrifuge
- Vortex
- Squirt bottle, manifold dispenser, or automated microplate washer
- Absorbent paper for blotting the microtiter plate
- 50-300ul multi-channel micropipette
- Pipette tips
- Single-channel micropipette with different ranges
- 100ml and 500ml graduated cylinders
- Deionized or distilled water
- Timer
- Test tubes for dilution
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數(shù)據(jù)處理：
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貨期：
3-5 working days
引用文獻
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The involvement of long noncoding RNA APOA1-AS in the pathogenesis of preeclampsia
HY Yang,Human & experimental toxicology,2023
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Fructose Consumption Affects Placental Production of H2S: Impact on Preeclampsia-Related Parameters
X Zhao,Nutrients,2024
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Dendrobium nobile Lindley and its bibenzyl component moscatilin are able to protect retinal cells from ischemia/hypoxia by dowregulating placental growth factor and upregulating Norrie disease protein
Wen-Haur Chao.et al,BMC Complementary and Alternative Medicine,2018
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Celastrol attenuates symptoms of preeclampsia in rats by inhibiting matrix metalloproteinase-9
Sha Xiao.et al,European Journal of Pharmacology,2018
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Ferulic acid alleviates symptoms of preeclampsia in rats by upregulating vascular endothelial growth factor
Gong W.et al,Clin Exp Pharmacol Physiol. ,2017
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The effects of sildenafil citrate on urinary podocin and nephrin mRNA expression in an l-NAME model of pre-eclampsia
Sooraj Baijnath.et al,Molecular and Cellular Biochemistry,2016
靶點詳情
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功能：
Growth factor active in angiogenesis and endothelial cell growth, stimulating their proliferation and migration. It binds to the receptor FLT1/VEGFR-1. Also promotes cell tumor growth.
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基因功能參考文獻：
- Studied the influence of dendrobium nobile Lindley (DNL) extract and its bibenzyl component moscatilin on the expression of placental growth factor (PLGF) and Norrie disease protein (NDP) in hypoxia model in retinal cells. Found DNL and moscatilin are able to protect against retinal ischemic/hypoxic changes respectively by downregulating PLGF and upregulating NDP. PMID: 29933759
- this paper show that knockdown of PLGF mitigates damage in hyperoxic lung by blocking of NFkappaB signaling PMID: 27280587
- PLGF triggers focal adhesion disassembly in alveolar type II cells via inhibiting the activation of focal adhesion kinase. PMID: 25515701
- PlGF had a positive feedback regulation with 15-lipoxygenase expression and 15-hydroxyeicosatetraenoic acid generation. PMID: 24001991
- placental gene expression is modulated by gestational conditions that might disrupt the fetal growth PMID: 23691181
- PlGF repairs myocardial ischemia through mechanisms of angiogenesis, cardioprotection and recruitment of myo-angiogenic competent marrow progenitors PMID: 21969865
- augmentation of brain Placental growth factor is associated with development of epilepsy. PMID: 22079325
- PLGF expression in the lung tissue in rats with paraquat-induced pulmonary fibrosis is upregulated. PMID: 17997886
- This is the first demonstration that sustained intraocular PGF production induces vascular and retinal changes similar to those observed in the early stages of diabetic retinopathy. PMID: 21408222
- Intramyocardial injections of autologous myoblasts overexpressing placental growth factor improved cardiac function, attenuated adverse cardiac remodeling, induced angiogenesis, and probably enhanced survival of grafted myoblasts. PMID: 21329947
- This study shows for the first time the placental growth factor upregulation in regenerating livers, which is related to hypoxia stimulation and liver growth. PMID: 21088600
- Endothelial cell-released H2O2 acts as a positive regulator of placenta growth factor gene and protein expression in vascular smooth muscle cells. PMID: 21123739
- The ARPE19 human junctions in culture are modulated by VEGF through VEGFR-1 but not through VEGFR-2. PMID: 18421240
- The levels of PlGF-2 in non-malformed embryos of diabetic gestation and their placentas were significantly (P < 0.05) lower than the average of controls. PMID: 19048427
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亞細胞定位：
Secreted.
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蛋白家族：
PDGF/VEGF growth factor family
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數(shù)據(jù)庫鏈接：
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