Rat periostin/osteoblast specific factor 2 (POSTN) ELISA kit

產(chǎn)品詳情

說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

大鼠骨膜蛋白/成骨細(xì)胞特異性因子2(POSTN)酶聯(lián)免疫試劑盒（CSB-EL018381RA）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的POSTN含量。POSTN是一個(gè)靶點(diǎn)。它與多種生理和病理過程相關(guān)，在腫瘤發(fā)生、發(fā)展中可能起重要作用。研究機(jī)制上，關(guān)注其在細(xì)胞外基質(zhì)中的功能，以及與腫瘤細(xì)胞增殖、遷移、侵襲等的關(guān)聯(lián)，有望為腫瘤等疾病的治療提供新方向。試劑盒檢測(cè)范圍為31.25 ng/mL-2000 ng/mL，適用于骨骼生物學(xué)研究、骨損傷修復(fù)機(jī)制探索、纖維化疾病模型建立及腫瘤微環(huán)境相關(guān)研究等科研領(lǐng)域，為體外定量分析POSTN在生理或病理?xiàng)l件下的動(dòng)態(tài)變化提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

POSTN
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, tissue homogenates
檢測(cè)范圍：

31.25 ng/mL-2000 ng/mL
靈敏度：

7.81 ng/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長：

450 nm
研究領(lǐng)域：

Cardiovascular
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat POSTN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	86-96
1:2	Average %	104
	Range %	98-108
1:4	Average %	90
	Range %	85-94
1:8	Average %	98
	Range %	92-101

回收率：

The recovery of rat POSTN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	91	84-95
EDTA plasma (n=4)	98	90-103

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.