Rat matrix metalloproteinase 3,MMP-3 ELISA kit

Rat matrix metalloproteinase 3,MMP-3 ELISA kit

產品詳情

引用文獻

產品評價

靶點詳情

產品詳情

引用文獻

產品評價

相關產品

靶點詳情

Most popular with customers

產品中心

信號通路

技術信息

聯系信息

文獻引用 (8) 說明書下載

中文名稱：

大鼠基質金屬蛋白酶3(MMP-3)酶聯免疫試劑盒
貨號：

CSB-E07410r
規格：

96T/48T
價格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

產品詳情
產品評價
引用文獻
相關產品
靶點詳情

產品描述：

大鼠基質金屬蛋白酶3(MMP-3)酶聯免疫試劑盒（CSB-E07410r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養上清液、組織勻漿樣本中的MMP3含量。MMP3，即基質金屬蛋白酶3，是一種在多種生理和病理過程中發揮關鍵作用的酶類分子。它在炎癥性疾病、腫瘤、動脈粥樣硬化和神經系統疾病中扮演重要角色，能夠降解多種基質成分，并激活其他MMPs，參與組織重塑和疾病進程。研究顯示，MMP3的基因多態性與某些疾病的遺傳易感性相關，如食管鱗癌。試劑盒檢測范圍為1.56 pg/mL-100 pg/mL，本產品可廣泛應用于基礎醫學研究領域，如關節炎動物模型中的基質代謝研究、藥物干預下MMP - 3表達水平動態監測，或腫瘤微環境中蛋白酶活性評估等實驗場景，為組織重塑機制探索及病理模型構建提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

Mmp3 ELISA Kit; Stromelysin-1 ELISA Kit; SL-1 ELISA Kit; EC 3.4.24.17 ELISA Kit; Matrix metalloproteinase-3 ELISA Kit; MMP-3 ELISA Kit; PTR1 protein ELISA Kit; Transin-1 ELISA Kit
縮寫：

MMP3
Uniprot No.：

P03957
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, cell culture supernates, tissue homogenates
檢測范圍：

1.56 pg/mL-100 pg/mL
靈敏度：

0.39 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Cancer
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat MMP-3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	90
	Range %	87-93
1:200	Average %	94
	Range %	90-98
1:400	Average %	95
	Range %	91-99
1:800	Average %	92
	Range %	88-96

回收率：

The recovery of rat MMP-3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	85	81-89
EDTA plasma (n=4)	94	90-98

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
100	2.569	2.547	2.558	2.429
50	2.236	2.213	2.225	2.096
25	1.868	1.829	1.849	1.720
12.5	1.367	1.387	1.377	1.248
6.25	0.840	0.889	0.865	0.736
3.13	0.377	0.387	0.382	0.253
1.56	0.247	0.259	0.253	0.124
0	0.128	0.130	0.129

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

Ruxolitinib Delays Nucleus Pulposus Cell Senescence in Rat Intervertebral Discs H Hao, W Liang, S Zhang, X Cai, A Yakefu, S Gao,JOR spine,2025
Dietary Supplementation with Palmitoyl-Glucosamine Co-Micronized with Curcumin Relieves Osteoarthritis Pain and Benefits Joint Mobility E Gugliandolo,Animals,2020
Safety and efficacy of a new micronized formulation of the ALIAmide palmitoylglucosamine in preclinical models of inflammation and osteoarthritis pain Cordaro M, et al,Arthritis Research & Therapy,2019
Venlafaxine alleviates complete Freund's adjuvant-induced arthritis in rats: Modulation of STAT-3/IL-17/RANKL axis Kamel KM, et al,Life Sciences,2019
Novel Anti-arthritic Mechanisms of Polydatin in Complete Freund’s Adjuvant-Induced Arthritis in Rats: Involvement of IL-6, STAT-3, IL-17, and NF-кB Kamel M. Kamel.et al,Inflammation,2018
A novel composite formulation of palmitoylethanolamide and quercetin decreases inflammation and relieves pain in inflammatory and osteoarthritic pain models Britti D.et al,BMC Vet Res.,2017
Adelmidrol, in combination with hyaluronic acid, displays increased anti-inflammatory and analgesic effects against monosodium iodoacetate-induced osteoarthritis in rats Rosanna Di Paola.et al,Arthritis Research & Therapy,2016
Matrix metalloproteinases are possible targets inmonocrotaline-induced pulmonary hypertension: investigationof anti-remodeling effects of alagebrium and everolimus Atl.et al,Anatolian journal of cardiology,2016

收起更多

功能：

Can degrade fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. Activates procollagenase.
亞細胞定位：

Secreted, extracellular space, extracellular matrix. Secreted.
蛋白家族：

Peptidase M10A family
數據庫鏈接：

UniGene: Rn.32086

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.