Rat heart fatty acid binding protein(h-FABP)ELISA Kit

產品詳情

文獻引用 (3) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

大鼠心型脂肪酸結合蛋白(h-FABP)酶聯免疫試劑盒（CSB-E16184r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養上清液、組織勻漿樣本中的FABP3含量。FABP3即脂肪酸結合蛋白3，在心肌、骨骼肌等組織中高表達。它可結合并轉運長鏈脂肪酸，參與細胞內的脂質代謝。目前在心血管疾病等方面是研究熱點，其機制研究有助于深入了解疾病發生發展，也為相關疾病診斷和治療提供新方向。試劑盒檢測范圍為15.6 pg/mL-1000 pg/mL，基礎科研領域，如心肌損傷動物模型評估、心血管疾病病理機制研究、藥物干預效果分析等場景，可兼容離體培養細胞分泌蛋白檢測及心肌組織勻漿樣本分析本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

Fabp3Fatty acid-binding protein ELISA kit; heart ELISA kit; Fatty acid-binding protein 3 ELISA kit; Heart-type fatty acid-binding protein ELISA kit; H-FABP ELISA kit
縮寫：

FABP3
Uniprot No.：

P07483
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, cell culture supernates, tissue homogenates
檢測范圍：

15.6 pg/mL-1000 pg/mL
靈敏度：

3.9 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Signal Transduction
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat h-FABP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	85
	Range %	84-92
1:2	Average %	96
	Range %	91-102
1:4	Average %	84
	Range %	81-88
1:8	Average %	94
	Range %	90-98

回收率：

The recovery of rat h-FABP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	87-95
EDTA plasma (n=4)	103	95-108

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

FABP are thought to play a role in the intracellular transport of long-chain fatty acids and their acyl-CoA esters.
基因功能參考文獻：
1. Significant down-regulation of heart-type fatty acid binding protein after myocardial ischemia might play an important role in myocardial injury and energy metabolism disorder. PMID: 21155221
2. H-FABP might have potential to be an early and quantitative parameter of clinical severity and prognosis in CO poisoning. PMID: 20375122
3. A complex interrelationship between glucose and fatty acids in the control of FABP gene expression; FABP3 and 5 may play a role in insulin secretion. PMID: 21099311
4. rat myocardial FABP gene maps to chromosome 5q36 and was detected in a preparation of total RNA isolated from adult rat heart PMID: 10561574
亞細胞定位：

Cytoplasm.
蛋白家族：

Calycin superfamily, Fatty-acid binding protein (FABP) family
組織特異性：

Heart, but also skeletal muscle, kidney, brain and mammary gland.
數據庫鏈接：

KEGG: rno:79131

STRING: 10116.ENSRNOP00000017325

UniGene: Rn.32566