Rat apolipoprotein A1 (Apo-A1) ELISA Kit

Rat apolipoprotein A1 (Apo-A1) ELISA Kit

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中文名稱：

大鼠載脂蛋白A1(Apo-A1)酶聯(lián)免疫試劑盒
貨號(hào)：

CSB-E08105r
規(guī)格：

96T/48T
價(jià)格：

￥3900/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產(chǎn)品描述：

大鼠載脂蛋白A1(Apo-A1)酶聯(lián)免疫試劑盒（CSB-E08105r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的APOA1含量。APOA1是重要靶點(diǎn)。它是高密度脂蛋白（HDL）的主要載脂蛋白，與膽固醇逆轉(zhuǎn)運(yùn)相關(guān)，有助于將外周組織膽固醇轉(zhuǎn)運(yùn)回肝臟代謝。研究發(fā)現(xiàn)其在動(dòng)脈粥樣硬化等疾病進(jìn)程中發(fā)揮關(guān)鍵作用，通過提高其水平或活性有望改善脂質(zhì)代謝、防治相關(guān)疾病。試劑盒檢測范圍為0.14 ng/ml-100 ng/ml.，靈敏度為0.14 ng/ml.。本試劑盒適用于基礎(chǔ)科研中對大鼠模型脂代謝機(jī)制的研究，例如通過檢測不同實(shí)驗(yàn)條件下（如基因干預(yù)、飲食調(diào)控或藥物處理）血清或組織中的Apo - A1濃度變化，評(píng)估心血管疾病模型的病理進(jìn)程或干預(yù)效果。為探索Apo - A1在脂質(zhì)穩(wěn)態(tài)及代謝性疾病中的功能提供高效研究工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

Apoa1Apolipoprotein A-I ELISA kit; Apo-AI ELISA kit; ApoA-I ELISA kit; Apolipoprotein A1) [Cleaved into: Proapolipoprotein A-I ELISA kit; ProapoA-I)] ELISA kit
縮寫：

APOA1
Uniprot No.：

P04639
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

0.14 ng/ml-100 ng/ml.
靈敏度：

0.14 ng/ml.
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Cardiovascular
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat Apo-A1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1000	Average %	94
	Range %	88-108
1:2000	Average %	97
	Range %	91-105
1:4000	Average %	103
	Range %	98-108
1:8000	Average %	93
	Range %	86-98

回收率：

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml

OD1

OD2

Average

Corrected

100

2.340

2.434

2.387

2.275

33.33

1.892

1.867

1.880

1.768

11.11

1.157

1.128

1.142

1.031

3.70

0.625

0.639

0.632

0.521

1.23

0.297

0.311

0.304

0.192

0.41

0.188

0.193

0.190

0.079

0.14

0.134

0.140

0.137

0.025

0.108

0.115

0.112

數(shù)據(jù)處理：

ELISA操作視頻及標(biāo)曲繪制軟件下載
貨期：

3-5 working days

Hypolipidemic and anti-inflammatory effects of aorta and heart tissues of cattle and pigs in the atherosclerosis rat model Chernukha IM.et al,Anim Sci J,2018
Effect of n-3 PUFA supplementation at different EPA: DHA ratios on the spontaneously hypertensive obese rat model of the metabolic syndrome Molinar-Toribio E et al,Br J Nutr,2015

最新研究進(jìn)展：

APOA1是一種高密度脂蛋白(HDL)的主要成分，參與調(diào)節(jié)膽固醇代謝和清除血管內(nèi)的膽固醇。最新研究表明，APOA1可以抑制乳腺癌的惡性轉(zhuǎn)化和肺癌細(xì)胞的增殖，可能成為腫瘤治療的新靶點(diǎn)。此外，APOA1水平與糖尿病、心血管疾病和肝病等多種疾病相關(guān)。
功能：

Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activates spermatozoa motility.
基因功能參考文獻(xiàn)：
1. both the route of administration and the formulation of apoA-I peptide significantly affect its pharmacokinetics and pharmacodynamics. PMID: 27881716
2. ABCA1 is diminished and ABCG1 is increased by N-acetylcysteine, which counteracts the reduction in apo A-I-mediated cholesterol efflux PMID: 25305669
3. Apolipoprotein A-I induces tubulin phosphorylation in association with cholesterol release in fetal rat astrocytes. PMID: 24814947
4. ApoA-I may be a protective blood-borne factor involved in the remote ischemic preconditioning mechanism. PMID: 24155931
5. It maintains cholesterol homeostasis. PMID: 24117066
6. on neonatal rat ventricular cardiomyocytes, the aldosterone-dependent chronotropic effects of antiapoA-1 IgG involve the nongenomic activation of L-type calcium channels PMID: 22253414
7. translocation of cholesterol and phospholipid into the cytosol is related to the apo A-I-mediated HDL assembly in astrocytes through functional association with caveolin-1 and a cyclosporin A-sensitive cyclophilin protein(s). PMID: 11773045
8. ABCA1-dependent cholesterol mobilization to apoA-I increased new cholesterol synthesis, indicating depletion of the regulatory pool of hepatocyte cholesterol during HDL formation PMID: 14993246
9. The association of CLPP with microtubules is mediated by caveolin-1, induced by apoA-I and regulates intracellular cholesterol trafficking for assembly of cellular lipids to apoA-I-HDL. PMID: 16606364
10. Data show that a complex of apolipoprotein A-I and pregnenolone modulate the rate of protein biosynthesis in liver cells. PMID: 18457017
11. ApoA-I reduced I/R-induced inflammatory responses, decreased renal microscopic damage and improved renal function. ApoA-I protects kidney from I/R injury by inhibiting inflammatory cytokines release and neutrophil infiltration and activation. PMID: 18535924
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亞細(xì)胞定位：

Secreted.
蛋白家族：

Apolipoprotein A1/A4/E family
組織特異性：

Major protein of plasma HDL, also found in chylomicrons.
數(shù)據(jù)庫鏈接：

KEGG: rno:25081

STRING: 10116.ENSRNOP00000065206

UniGene: Rn.10308

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.