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中文名稱：大鼠晚期糖基化終末產(chǎn)物(AGEs)酶聯(lián)免疫試劑盒
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貨號：CSB-E09413r
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規(guī)格：96T/48T
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價格：￥3900/￥2500
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促銷：
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其他：
產(chǎn)品詳情
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產(chǎn)品描述：大鼠晚期糖基化終末產(chǎn)物(AGEs)酶聯(lián)免疫試劑盒（CSB-E09413r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的AGEs含量。AGEs即晚期糖基化終末產(chǎn)物，在高血糖等情況下形成并不斷累積。其研究機制主要是AGEs與受體結(jié)合后激活多條信號通路，引發(fā)氧化應(yīng)激、炎癥反應(yīng)等，與糖尿病并發(fā)癥、神經(jīng)退行性疾病等多種疾病的發(fā)生發(fā)展密切相關(guān)。試劑盒檢測范圍為3.9 μg/mL-250 μg/mL，適用于糖尿病動物模型研究、衰老相關(guān)機制探索、氧化應(yīng)激水平評估等科研領(lǐng)域，尤其適合在心血管疾病、神經(jīng)退行性疾病等慢性病理模型的建立與驗證中，通過血清、血漿或組織樣本的動態(tài)監(jiān)測揭示AGEs的生物學(xué)作用。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
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縮寫：AGEs
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種屬：Rattus norvegicus (Rat)
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樣本類型：serum, plasma, tissue homogenates
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檢測范圍：3.9 μg/mL-250 μg/mL
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靈敏度：0.97 μg/mL
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反應(yīng)時間：1-5h
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樣本體積：50-100ul
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檢測波長：450 nm
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研究領(lǐng)域：Immunology
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測定原理：quantitative
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測定方法：Sandwich
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精密度：
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度：
To assess the linearity of the assay, samples were spiked with high concentrations of rat AGEs in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:5 Average % 86 Range % 84-95 1:10 Average % 95 Range % 91-100 1:20 Average % 85 Range % 81-89 1:40 Average % 93 Range % 89-96 -
回收率：
The recovery of rat AGEs spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 91 87-96 EDTA plasma (n=4) 95 90-100 -
標(biāo)準(zhǔn)曲線：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. 
μg/ml OD1 OD2 Average Corrected 250 2.194 2.286 2.240 2.084 125 1.764 1.789 1.777 1.621 62.5 1.270 1.256 1.263 1.107 31.2 0.921 0.975 0.948 0.792 15.6 0.455 0.465 0.460 0.304 7.8 0.297 0.308 0.303 0.147 3.9 0.248 0.233 0.241 0.085 0 0.161 0.150 0.156 -
數(shù)據(jù)處理：
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貨期：3-5 working days
引用文獻
- Antioxidant and Hepatoprotective Effects of Moringa oleifera-mediated Selenium Nanoparticles in Diabetic Rats. AA Ahmad Tarmizi, NN Nik Ramli,F1000Research,2025
- Okanin alleviates symptoms of nociceptive-like responses in diabetic peripheral neuropathy in type 1 diabetic Wistar rats by regulating the AGEs/NF-κB/Nrf-2 pathway MR Ganie, N Khan, M Shukla, S Sood, S Devi,Journal of Pharmacological Sciences,2024
- Carthamus tinctorius L. (Safflower) Flower Extract Attenuates Hepatic Injury and Steatosis in a Rat Model of Type 2 Diabetes Mellitus via Nrf2-Dependent Hypoglycemic, Antioxidant, and Hypolipidemic Effects NS Alshareef, SA AlSedairy, LN Al-Harbi,Antioxidants,2024
- Comparative Efficacy of Low-Carbohydrate and Ketogenic Diets on Diabetic Retinopathy and Oxidative Stress in High-Fat Diet-Induced Diabetic Rats MT Jawharji, GM Alshammari, MA Binobead,Nutrients,2024
- Phloretamide Protects against Diabetic Kidney Damage and Dysfunction in Diabetic Rats by Attenuating Hyperglycemia and Hyperlipidemia, Suppressing NF-κβ, and Upregulating Nrf2 R Al,Pharmaceutics,2024
- Renoprotective impact of Dapagliflozin and Mulberry extracts toward Fr-STZ induced diabetic nephropathy in rats: Biochemical and Molecular aspects EM Gad,researchsquare,2023
- Neuroprotective Activity of Eriodictyol Against Streptozotocin-Induced Diabetic Peripheral Neuropathy in Wistar Rats by Targeting Wnt/β-Catenin Pathway R Malik,researchsquare,2023
- A standardized pomegranate fruit extract ameliorates thioacetamide-induced liver fibrosis in rats via AGE-RAGE-ROS signaling HM Abouelezz,Heliyon,2023
- Role of Klotho and AGE/RAGE-Wnt/β-Catenin Signalling Pathway on the Development of Cardiac and Renal Fibrosis in Diabetes B Martín-Carro,International Journal of Molecular Sciences,2023
- DL0410 Alleviates Memory Impairment in D-Galactose-Induced Aging Rats by Suppressing Neuroinflammation via the TLR4/MyD88/NF-κB Pathway B Zhang,Oxid Med Cell Longev,2021
- Activation of RAGE-dependent endoplasmic reticulum stress associates with exacerbated post-myocardial infarction ventricular arrhythmias in diabetes Z Liu,American Journal of Physiology-Endocrinology and Metabolism,2021
- Astragaloside IV Protects Against Diabetic Nephropathy by Inhibiting Pyroptosis Based on NOX4/TXNIP/NLRP3 Pathway Y Zhang,Authorea,2020
- Transcriptomic Analysis Reveals the Protection of Astragaloside IV against Diabetic Nephropathy by Modulating Inflammation Y Zhang,Oxid Med Cell Longev,2020
- Standardised Extracts of Moringa Oleifera and Centella Asiatica Enhanced the Antioxidant Activity, Learning and Memory Effects by Inhibiting … H Jamari,Pertanika J. Sci. & Technol.,2020
- Effects and mechanisms of PSS-loaded nanoparticles on coronary microcirculation dysfunction in streptozotocin-induced diabetic cardiomyopathy rats Mao Y, et al,Biomedicine & Pharmacotherapy,2019
- Integrated proteomics, biological functional assessments and metabolomics reveal toosendanin induced hepatic energy metabolic disorders Yan X, et al,Chemical research in toxicology,2019
- Analysis of differentially expressed advanced glycation end product-modified proteins in diabetic rat kidney Jingfang Liu.et al,International Journal of Diabetes in Developing Countries,2018
- GS-E3D, a new pectin lyase-modified red ginseng extract, inhibited diabetes-related renal dysfunction in streptozotocin-induced diabetic rats Kim CS.et al,BMC Complement Altern Med.,2017
- Antiglycation and Hypolipidemic Effects of Polyphenols from Zingiber officinale Roscoe (Zingiberaceae) in Streptozotocin-Induced Diabetic Rats Mutiu I Kazeem et al,Tropical Journal of Pharmaceutical Research January,2015
- Advanced glycation end products (AGE) induce the receptor for AGE in the colonic mucosa of azoxymethane-injected Fischer 344 rats fed with a high-linoleic acid and high-glucose diet /,Journal of Gastroenterology,2012
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相關(guān)問答
■ 常見問題解答
Q:
我想從貴公司訂購“rat advanced glycation end products (AGEs) ELISA Kit (#CSB-E09413r)”，但我想了解以下信息： Q1,是否有數(shù)據(jù)說明為什么我們可以根據(jù)宿主物種選擇AGEs Elisa試劑盒？由于AGEs沒有特異性表位，我認為宿主物種并不是確定AGEs抗體特異性的關(guān)鍵因素。 Q2.如果我使用大鼠AGEs ELISA kit來分析小鼠組織樣本，很難保證該試劑盒的特異性或數(shù)據(jù)質(zhì)量問題嗎？
A:
A1：AGEs是一系列物質(zhì)的通稱，而不是一個單一的物質(zhì)。它們具有相同的抗原決定簇。我們之所以有不同物種試劑盒，是不同物種的試劑盒采用原料特性不同，抗基質(zhì)干擾、適用性也不同。這個CSB-E09413r試劑盒（大鼠AGEs ELISA試劑盒）應(yīng)用了小鼠單克隆抗體和山羊多克隆抗體。而這個CSB-E09414m試劑盒（小鼠AGEs ELISA試劑盒）應(yīng)用了大鼠單克隆抗體和山羊多克隆抗體。因此我們建議用戶使用相應(yīng)物種的ELISA試劑盒進行檢測。
A2：這個試劑盒與人和小鼠AGEs重組蛋白沒有交叉反應(yīng)。我們用這個試劑盒測試了小鼠血樣，沒有檢測到任何數(shù)值。我們不建議進行跨物種檢測。
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