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Rat Thyroid-Peroxidase,TPO ELISA Kit

文獻引用 (1) 說明書下載

產品詳情

產品描述：

大鼠甲狀腺過氧化物酶(TPO)酶聯免疫試劑盒（CSB-E08352r）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的TPO含量。TPO即血小板生成素，其作為造血調控因子，在血小板生成過程中有關鍵作用。研究機制主要圍繞它結合并激活跨膜TPO受體，啟動信號傳導途徑，促進造血干細胞向巨核系祖細胞分化、增殖及成熟，進而產生血小板。試劑盒檢測范圍為2.5 ng/mL-40ng/mL，適用于甲狀腺相關疾病的動物模型研究、環境毒理評估中甲狀腺功能干擾物的篩選，以及甲狀腺自身抗體作用機制等基礎科研領域本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

Tpo ELISA Kit; Thyroid peroxidase ELISA Kit; TPO ELISA Kit; EC 1.11.1.8 ELISA Kit
縮寫：

TPO
Uniprot No.：

P14650
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, tissue Homogenates
檢測范圍：

2.5 ng/mL-40ng/mL
靈敏度：

2.5 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Cancer
測定原理：

quantitative
測定方法：

Competitive

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat TPO in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	93
	Range %	90-97
1:2	Average %	95
	Range %	84-99
1:4	Average %	90
	Range %	82-100
1:8	Average %	95
	Range %	89-100

回收率：

The recovery of rat TPO spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	87-102
EDTA plasma (n=4)	94	89-100

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Iodination and coupling of the hormonogenic tyrosines in thyroglobulin to yield the thyroid hormones T(3) and T(4).
亞細胞定位：

Membrane; Single-pass type I membrane protein.
蛋白家族：

Peroxidase family, XPO subfamily
數據庫鏈接：

STRING: 10116.ENSRNOP00000006526

UniGene: Rn.233736