Rat Rheumatoid Factor(RF) ELISA Kit

Rat Rheumatoid Factor(RF) ELISA Kit

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中文名稱：

大鼠類風濕因子(RF)酶聯免疫試劑盒
貨號：

CSB-E13666r
規格：

96T/48T
價格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產品描述：

大鼠類風濕因子(RF)酶聯免疫試劑盒（CSB-E13666r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿樣本中的RF含量。大鼠類風濕因子（RF）是一種自身免疫性疾病類風濕關節炎（RA）的標志物，其研究機制涉及免疫系統的異常激活，包括抗原識別、T細胞介導的炎癥反應和軟骨破壞。通過建立動物模型，研究者可以模擬人類RA的病理特征，從而探索發病機制和開發新的治療策略。試劑盒檢測范圍為15.6 mIU/mL-1000 mIU/mL，該產品適用于科研場景下大鼠血清或血漿樣本的RF水平檢測，如關節炎動物模型的炎癥程度分析、免疫調節劑篩選實驗或基礎醫學研究中自身免疫反應動態監測，為研究者提供穩定可靠的實驗工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

N/A
縮寫：

RF
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma
檢測范圍：

15.6 mIU/mL-1000 mIU/mL
靈敏度：

3.9 mIU/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat RF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:5	Average %	104
	Range %	96-108
1:10	Average %	94
	Range %	91-98
1:20	Average %	102
	Range %	98-106
1:40	Average %	88
	Range %	82-96

回收率：

The recovery of rat RF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	83-95
EDTA plasma (n=4)	99	92-107

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

mIU/ml	OD1	OD2	Average	Corrected
1000	2.646	2.545	2.596	2.443
500	1.975	2.076	2.026	1.873
250	1.284	1.308	1.296	1.143
125	0.751	0.762	0.757	0.604
62.5	0.512	0.501	0.507	0.354
31.2	0.353	0.342	0.348	0.195
15.6	0.285	0.281	0.283	0.130
0	0.153	0.152	0.153

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

Preconditioning of bone marrow mesenchymal stem cells with sodium hydrosulfide enhances their therapeutic potential in type II collagen–induced arthritis rat model SM El-Sayed, HH Ahmed, HA Aglan,Naunyn-Schmiedeberg's Archives of Pharmacology,2025
Norisoboldine Reduces Arthritis Severity by Attenuating Inflammation, Oxidative Stress, and Extracellular Matrix Degradation in a Rat Model of Rheumatoid Arthritis Y Wang, X Zhao, J Wang, X Zhu,Journal of Inflammation Research,2024
Visnagin alleviates rheumatoid arthritis via its potential inhibitory impact on malate dehydrogenase enzyme: in silico, in vitro, and in vivo studies AA Khamis, AH Sharshar, TM Mohamed,Genes & Nutrition,2024
Investigating the therapeutic potential of sinomenine in rheumatoid arthritis: anti-inflammatory, antioxidant, and immunomodulatory mechanisms J Li,Naunyn-Schmiedeberg's archives of pharmacology,2023
Cerastes snake venom as a promising approach in the management of complete Freund's adjuvant-induced rheumatoid arthritis in rats: Involvement of RANKL and JAK/STAT pathway NS Soliman,Journal of ethnopharmacology,2023
Anti-arthritic activity of solvent extracts of the bulbs of Crinum pedunculatum R.Br. P Doe,Pharmaceutical Sciences Asia,2022
CS12192, a Novel JAK3/JAK1/TBK1 Inhibitor, Synergistically Enhances the Anti-Inflammation Effect of Methotrexate in a Rat Model of Rheumatoid Arthritis Z Fang,International journal of molecular sciences,2022
Diosmin and Trolox Have Anti-Arthritic, Anti-Inflammatory and Antioxidant Potencies in Complete Freund’s Adjuvant-Induced Arthritic Male Wistar Rats: Roles of NF-κB, iNOS, Nrf2 and MMPs HH Shaaban,Antioxidants,2022
Leurieus quinquestriatus scorpion venom ameliorates adjuvant-induced arthritis in rats: Modulating JAK/STAT/RANKL signal transduction pathway NS Soliman,International immunopharmacology,2022
Anti-inflammatory effects of evening primrose and extra virgin olive oil on arthritc rats BM Mahmoud,Advances in Animal and Veterinary Sciences,2021
The Enhanced Beneficial Effect of Extra Virgin Olive Oil Over Evening Primrose Oil on Oxidative Stress, and Liver Function in Male Arthritic Rats S Zhang,Journal of Pharmaceutical Research International,2021
TDZD-8 Suppresses Rheumatoid Arthritis Induced by Collagen Type II in Rats: Potential Role of Apoptosis and Inflammation AF Dawood,International Journal of Morphology,2021
Activation of activin/Smad2 and 3 signaling pathway and the potential involvement of endothelial?mesenchymal transition in the valvular damage due to rheumatic heart disease S Xian,Molecular Medicine Reports,2020
Inhibition of GSK3β protects against collagen type II┸\induced arthritis associated with a decrease in synovial leukocyte infiltration and inhibition of endoplasmic … NM Alzamil,Clin Exp Pharmacol Physiol,2020
Inhibition of glycogen synthase kinase-3β protects against collagen type II-induced arthritis associated with the inhibition of miR155/24 and inflammation and … AF Dawood,Arch. Physiol. Biochem,2020
A novel mPGES-1 inhibitor alleviates inflammatory responses by downregulating PGE2 in experimental models Lee HH, et al,Prostaglandins & Other Lipid Mediators,2019
Effect of mandarin peel extract on experimentally induced arthritis in male rats Sakr HI,ARCHIVES OF PHYSIOLOGY AND BIOCHEMISTRY,2019
Ulva lactuca hydroethanolic extract suppresses experimental arthritis via its anti-inflammatory and antioxidant activities Osama M. Ahmed.et al,Beni-Suef University Journal of Basic and Applied Sciences,2017
Standardized ethyl acetate fraction from the roots of Brassica rapa attenuates the experimental arthritis by down regulating inflammatory responses and inhibiting NF-kB activation. Shin JS et al,Food Chem Toxicol,2014

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.