Rat Resistin ELISA Kit

產品詳情

文獻引用 (2) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

大鼠抵抗素(Resistin)酶聯免疫試劑盒（CSB-E06885r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的Resistin含量。Resistin 是一種脂肪細胞因子，它與肥胖、胰島素抵抗和炎癥等密切相關。研究發現，其水平在肥胖個體中往往升高。機制上，它可能通過干擾胰島素信號通路、激活炎癥信號等，在代謝紊亂及相關疾病進程中發揮重要作用。試劑盒檢測范圍為125 pg/mL-8000 pg/mL，適用于科研領域中對大鼠代謝疾病模型（如2型糖尿病、肥胖癥）的機制研究、藥物干預效果評估或Resistin表達調控實驗，可通過檢測不同生理病理狀態下血清、組織等樣本的Resistin水平，揭示其在能量代謝與炎癥反應中的作用。試劑盒提供完整組分及操作說明書，確保實驗穩定性和重復性，滿足基礎研究與生物標志物探索需求。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

Resistin
Uniprot No.：

Q8K4J7
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

125 pg/mL-8000 pg/mL
靈敏度：

31.25 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Cardiovascular
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat resistin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	88-96
1:2	Average %	96
	Range %	91-101
1:4	Average %	95
	Range %	90-101
1:8	Average %	94
	Range %	90-98

回收率：

The recovery of rat resistin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	85-94
EDTA plasma (n=4)	99	95-103

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.