Rat Pro-opiomelanocortin(POMC) ELISA KIT

Rat Pro-opiomelanocortin(POMC) ELISA KIT

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中文名稱：

大鼠阿黑皮素原(POMC)酶聯(lián)免疫試劑盒
貨號：

CSB-E13569r
規(guī)格：

96T/48T
價格：

￥3800/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產(chǎn)品描述：

大鼠阿黑皮素原(POMC)酶聯(lián)免疫試劑盒（CSB-E13569r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的POMC含量。POMC（阿黑皮素原）是一種重要的神經(jīng)肽前體蛋白。它在人體能量平衡、代謝調(diào)節(jié)、神經(jīng)內(nèi)分泌等方面發(fā)揮關(guān)鍵作用。研究機制主要聚焦于其裂解產(chǎn)生的多種活性肽，如促黑素、促腎上腺皮質(zhì)激素等，參與調(diào)節(jié)食欲、應(yīng)激反應(yīng)等生理過程。試劑盒檢測范圍為7.8 pg/mL-500 pg/mL，適用于神經(jīng)內(nèi)分泌研究、肥胖機制探索、下丘腦 - 垂體軸功能分析等科研領(lǐng)域，特別適合評估實驗動物模型在藥物干預(yù)、代謝調(diào)控或應(yīng)激狀態(tài)下POMC的表達水平變化。為研究能量平衡、攝食行為及相關(guān)疾病的分子機制提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

Pomc ELISA kit; Pomc2Pro-opiomelanocortin ELISA kit; POMC ELISA kit; Corticotropin-lipotropin) [Cleaved into: NPP; Melanotropin gamma ELISA kit; Gamma-MSH); Potential peptide; Corticotropin ELISA kit; Adrenocorticotropic hormone ELISA kit; ACTH); Melanocyte-stimulating hormone alpha ELISA kit; Alpha-MSH ELISA kit; Melanotropin alpha); Corticotropin-like intermediary peptide ELISA kit; CLIP); Lipotropin beta ELISA kit; Beta-LPH); Lipotropin gamma ELISA kit; Gamma-LPH); Melanocyte-stimulating hormone beta ELISA kit; Beta-MSH ELISA kit; Melanotropin beta); Beta-endorphin; Met-enkephalin] ELISA kit
縮寫：

POMC
Uniprot No.：

P01194
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, cell culture supernates, tissue homogenates
檢測范圍：

7.8 pg/mL-500 pg/mL
靈敏度：

1.95 pg/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Cancer
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat POMC in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:5	Average %	99
	Range %	93-105
1:10	Average %	93
	Range %	90-97
1:20	Average %	95
	Range %	90-100
1:40	Average %	84
	Range %	80-88

回收率：

The recovery of rat POMC spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	91	87-95
EDTA plasma (n=4)	87	85-90

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
500	2.502	2.603	2.553	2.438
250	1.661	1.762	1.712	1.597
125	1.127	1.148	1.138	1.023
62.5	0.724	0.713	0.719	0.604
31.2	0.388	0.377	0.383	0.268
15.6	0.287	0.276	0.282	0.167
7.8	0.184	0.181	0.183	0.068
0	0.115	0.114	0.115

數(shù)據(jù)處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

功能：

Stimulates the adrenal glands to release cortisol.; Anorexigenic peptide. Increases the pigmentation of skin by increasing melanin production in melanocytes.; Increases the pigmentation of skin by increasing melanin production in melanocytes.; Endogenous orexigenic opiate.; Endogenous opiate.
亞細胞定位：

Secreted.
蛋白家族：

POMC family
組織特異性：

ACTH and MSH are produced by the pituitary gland.
數(shù)據(jù)庫鏈接：

STRING: 10116.ENSRNOP00000016976

UniGene: Rn.108195

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.