Rat Platelet-derived growth factor DD(PDGF-DD) ELISA kit
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中文名稱：大鼠血小板衍生生長因子DD(PDGF-DD) 酶聯(lián)免疫試劑盒
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貨號(hào)：CSB-EL017711RA
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規(guī)格：96T/48T
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價(jià)格：￥3600/￥2500
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其他：
產(chǎn)品詳情
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產(chǎn)品描述：大鼠血小板衍生生長因子DD(PDGF-DD) 酶聯(lián)免疫試劑盒（CSB-EL017711RA）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的PDGF-DD含量。PDGF - DD是一種血小板衍生生長因子異構(gòu)體。它在細(xì)胞的生長、增殖和遷移等過程中發(fā)揮重要作用。其研究機(jī)制主要聚焦于與受體結(jié)合后激活下游信號(hào)通路，影響細(xì)胞行為，在腫瘤、纖維化等疾病的發(fā)生發(fā)展中可能有潛在影響，是藥物研發(fā)的潛在靶點(diǎn)。試劑盒檢測范圍為0.156 ng/mL-10 ng/mL，適用于科研領(lǐng)域探究PDGF-DD在大鼠心血管疾病模型、器官纖維化機(jī)制、創(chuàng)傷修復(fù)實(shí)驗(yàn)中的動(dòng)態(tài)表達(dá)特征，通過血清或血漿樣本可系統(tǒng)性評(píng)估循環(huán)系統(tǒng)中PDGF-DD水平，而組織勻漿檢測則有助于解析特定器官或病變部位的局部蛋白表達(dá)模式，為相關(guān)分子機(jī)制研究提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
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縮寫：PDGF-DD
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種屬：Rattus norvegicus (Rat)
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樣本類型：serum, plasma, tissue homogenates
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檢測范圍：0.156 ng/mL-10 ng/mL
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靈敏度：0.039 ng/mL
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反應(yīng)時(shí)間：1-5h
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樣本體積：50-100ul
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檢測波長：450 nm
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研究領(lǐng)域：Others
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測定原理：quantitative
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測定方法：Sandwich
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精密度：
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度：
To assess the linearity of the assay, samples were spiked with high concentrations of rat PDGF-DD in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 86 Range % 80-90 1:2 Average % 89 Range % 85-96 1:4 Average % 90 Range % 84-96 1:8 Average % 92 Range % 86-98 -
回收率：
The recovery of rat PDGF-DD spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 96 92-103 EDTA plasma (n=4) 95 90-101 -
標(biāo)準(zhǔn)曲線：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. 
ng/ml OD1 OD2 Average Corrected 10 2.744 2.678 2.711 2.531 5 2.139 2.112 2.126 1.946 2.5 1.509 1.476 1.493 1.313 1.25 1.025 1.003 1.014 0.834 0.625 0.662 0.651 0.657 0.477 0.312 0.403 0.386 0.395 0.215 0.156 0.292 0.272 0.282 0.102 0 0.181 0.179 0.180 -
數(shù)據(jù)處理：
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貨期：3-5 working days
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