Mouse Platelet-derived growth factor DD(PDGF-DD) ELISA kit

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產(chǎn)品詳情

產(chǎn)品描述：

小鼠血小板衍生生長因子DD(PDGF-DD) 酶聯(lián)免疫試劑盒（CSB-EL017711MO）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的PDGF-DD含量。PDGF - DD是一種重要的靶點(diǎn)。它屬血小板衍生生長因子家族，在細(xì)胞增殖、遷移與血管生成等過程起關(guān)鍵作用。研究機(jī)制方面，主要聚焦其與受體結(jié)合后的信號傳導(dǎo)通路，以及在腫瘤、纖維化等疾病中的異常激活，為相關(guān)疾病治療提供新方向。試劑盒檢測范圍為0.156 ng/mL-10 ng/mL，適用于小鼠模型研究中PDGF-DD的動(dòng)態(tài)監(jiān)測，如探究其在組織修復(fù)、纖維化進(jìn)程或腫瘤微環(huán)境中的調(diào)控作用，支持心血管疾病、腫瘤生物學(xué)及再生醫(yī)學(xué)等領(lǐng)域的科研實(shí)驗(yàn)。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

PDGF-DD
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

0.156 ng/mL-10 ng/mL
靈敏度：

0.039 ng/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Others
測定原理：

quantitative
測定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse PDGF-DD in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	93
	Range %	89-96
1:2	Average %	102
	Range %	98-106
1:4	Average %	88
	Range %	84-93
1:8	Average %	100
	Range %	95-104

回收率：

The recovery of mouse PDGF-DD spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	86	83-89
EDTA plasma (n=4)	97	93-100

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.