Rat Nesfatin-1 ELISA Kit

產(chǎn)品詳情

文獻引用 (3) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

大鼠神經(jīng)肽Nesfatin-1酶聯(lián)免疫試劑盒（CSB-E14378r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的Nesfatin-1含量。Nesfatin-1是一種由核組蛋白2（NUCB2）衍生而來的肽段，主要在下丘腦和外周組織表達，具有抑制攝食、調(diào)節(jié)能量代謝、參與生長發(fā)育和胰島素抵抗等生物學(xué)功能。其研究機制涉及調(diào)節(jié)下丘腦黑皮質(zhì)素信號通路、影響食欲、降低血糖、促進胰島素分泌和調(diào)節(jié)糖代謝中酶的活性等方面。試劑盒檢測范圍為15.6 pg/mL-1000 pg/mL，適用于神經(jīng)生物學(xué)、代謝調(diào)控機制研究，以及肥胖相關(guān)動物模型的病理機制探索；為研究Nesfatin - 1在生理及病理過程中的作用提供可靠工具；適用于實驗室規(guī)模的樣本批量檢測本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

Nesfatin-1
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

15.6 pg/mL-1000 pg/mL
靈敏度：

3.9 pg/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Others
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat nesfatin-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	84
	Range %	80-92
1:2	Average %	89
	Range %	85-95
1:4	Average %	93
	Range %	89-99
1:8	Average %	95
	Range %	90-100

回收率：

The recovery of rat nesfatin-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	104	97-109
EDTA plasma (n=4)	97	93-102

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.