Human Nesfatin-1 ELISA Kit

Human Nesfatin-1 ELISA Kit

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中文名稱：

人神經(jīng)肽Nesfatin-1酶聯(lián)免疫試劑盒
貨號(hào)：

CSB-E15050h
規(guī)格：

96T/48T
價(jià)格：

￥3200/￥2500
促銷：
其他：

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產(chǎn)品描述：

人神經(jīng)肽Nesfatin-1酶聯(lián)免疫試劑盒（CSB-E15050h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的Nesfatin-1含量。Nesfatin-1是一個(gè)值得關(guān)注的靶點(diǎn)。它在體內(nèi)廣泛分布，與能量代謝、進(jìn)食調(diào)節(jié)等生理過程密切相關(guān)。目前研究聚焦于其作用機(jī)制，如通過與特定受體結(jié)合，影響神經(jīng)信號(hào)傳導(dǎo)，調(diào)節(jié)代謝相關(guān)激素分泌，為肥胖、代謝疾病治療帶來新思路。試劑盒檢測范圍為31.25 pg/mL-2000 pg/mL，適用于代謝疾病模型研究、肥胖相關(guān)神經(jīng)內(nèi)分泌機(jī)制探索，或通過檢測腦組織、外周血等樣本動(dòng)態(tài)觀察Nesfatin-1在應(yīng)激狀態(tài)下的表達(dá)變化，為研究能量代謝調(diào)控、神經(jīng)肽信號(hào)通路提供可靠工具，滿足基礎(chǔ)科研中對(duì)低豐度靶標(biāo)的高效檢測需求。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

Nesfatin-1
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

31.25 pg/mL-2000 pg/mL
靈敏度：

7.81 pg/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Others
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human Nesfatin-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:5	Average %	96
	Range %	80-105
1:10	Average %	99
	Range %	91-111
1:20	Average %	104
	Range %	92-112
1:40	Average %	93
	Range %	86-100

回收率：

The recovery of human Nesfatin-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	89-98
EDTA plasma (n=4)	97	90-107

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
2000	2.617	2.557	2.587	2.463
1000	2.205	2.356	2.281	2.157
500	1.636	1.767	1.702	1.578
250	1.043	1.058	1.051	0.927
125	0.609	0.642	0.626	0.502
62.5	0.352	0.346	0.349	0.225
31.25	0.226	0.243	0.235	0.111
0	0.122	0.125	0.124

數(shù)據(jù)處理：

ELISA操作視頻及標(biāo)曲繪制軟件下載
貨期：

3-5 working days

SERUM NESFATIN-1 LEVEL IN HEALTHY SUBJECTS WITH WEIGHT-RELATED ABNORMALITIES AND NEWLY DIAGNOSED PATIENTS WITH TYPE 2 DIABETES MELLITUS; A CASE-CONTROL STUDY S. Mirakhor Samani,ACTA ENDOCRINOLOGICA-BUCHAREST,2019
Increased plasma nesfatin-1 levels may be associated with corticosterone, IL-6, and CRP levels in patients with major depressive disorder Xia QR.et al,Clin Chim Acta,2018
Role of circulating nesfatin-1 in the underweight children with poor appetite Kaba S.et al,Eur Rev Med Pharmacol Sci.,2015
Association of nesfatin-1 level with body composition, dietary intake and resting metabolic rate in obese and morbid obese subjects Mirzaei K.et al,Diabetes Metab Syndr.,2015

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.