Rat Interleukin 5,IL-5 ELISA KIT

Rat Interleukin 5,IL-5 ELISA KIT

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中文名稱：

大鼠白介素5(IL-5)酶聯免疫試劑盒
貨號：

CSB-E07435r
規格：

96T/48T
價格：

￥3200/￥2500
促銷：
其他：

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產品描述：

大鼠白介素5(IL-5)酶聯免疫試劑盒（CSB-E07435r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養上清液、組織勻漿樣本中的IL5含量。IL-5 是一種重要的細胞因子，在嗜酸性粒細胞的生長、分化及存活中發揮關鍵作用。其過度表達與多種炎癥性疾病如哮喘、嗜酸性粒細胞增多癥等相關。研究聚焦于阻斷 IL-5 信號通路，以減少嗜酸性粒細胞數量，從而緩解相關疾病的炎癥癥狀。試劑盒檢測范圍為15.6 pg/mL-1000 pg/mL，適用于基礎科研領域中IL-5相關免疫調節機制研究，如構建哮喘動物模型時評估炎癥因子水平，或體外藥物篩選實驗中監測細胞因子分泌動態。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

Il5 ELISA Kit; Il-5 ELISA Kit; Interleukin-5 ELISA Kit; IL-5 ELISA Kit; B-cell growth factor II ELISA Kit; BCGF-II ELISA Kit; Cytotoxic T-lymphocyte inducer ELISA Kit; Eosinophil differentiation factor ELISA Kit; T-cell replacing factor ELISA Kit; TRF ELISA Kit
縮寫：

IL5
Uniprot No.：

Q08125
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, cell culture supernates, tissue homogenates
檢測范圍：

15.6 pg/mL-1000 pg/mL
靈敏度：

3.9 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	85
	Range %	82-91
1:2	Average %	92
	Range %	83-99
1:4	Average %	101
	Range %	93-107
1:8	Average %	96
	Range %	90-104

回收率：

The recovery of rat IL-5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	88	85-94
EDTA plasma (n=4)	94	90-100

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1000	2.407	2.457	2.432	2.268
500	1.768	1.736	1.752	1.588
250	1.065	1.098	1.082	0.918
125	0.704	0.735	0.720	0.556
62.5	0.448	0.469	0.459	0.295
31.2	0.323	0.300	0.312	0.148
15.6	0.221	0.229	0.225	0.061
0	0.161	0.167	0.164

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

Anti-allergic and anti-inflammatory effects of Gastrodia elata Blume extract in ovalbumin-induced asthma rat model JS Park, YS Lee, JW Seo, HJ Na,Laboratory Animal Research,2025
Glycerophospholipid metabolism licenses IgE-mediated mast cell degranulation Y Xia, P Bin, Y Zhou, M Zhao, J Zhang, W Zhong,Cell Reports,2025
Crude saponin from Korean red ginseng alleviates di (2-ethylhexyl) phthalate-induced urogenital damage via regulating p38 MAPK/NF-κB signaling JH Oh, SH Baek, HW Seo, SH Lee,Journal of Ginseng Research,2025
Stapokibart (CM310) targets IL-4Rα for the treatment of type 2 inflammation W Liu, Y Zhao, Y He, X Yan, J Yu, Q Song, L Zhang,iScience,2024
Orally administered solasodine, a steroidal glycoalkaloid, suppresses ovalbumin-induced exaggerated Th2-immune response in rat model of bronchial asthma P Arora,Chemico-biological interactions,2022
Lignosus rhinocerotis Cooke Ryvarden ameliorates airway inflammation, mucus hypersecretion and airway hyperresponsiveness in a murine model of asthma M Johnathan,Plos one,2021
Potential anti-inflammatory and immunomodulatory effects of carvacrol against ovalbumin-induced asthma in rats El-Din Y M E,Life Sciences,2019
In vitro-in vivo correlation of inhalable budesonide-loaded large porous particles for sustained treatment regimen of asthma Li J, et al,Acta Biomaterialia,2019
Sustained therapeutic efficacy of budesonide-loaded chitosan swellable microparticles after lung delivery: Influence of in vitro release, treatment interval and dose Lan Zhang.et al,Journal of Controlled Release,2018
Phytochemical profiles and inhibitory effects of Tiger Milk mushroom (Lignosus rhinocerus) extract on ovalbumin-induced airway inflammation in a rodent model of asthma. Johnathan M.et al,BMC Complement Altern Med.,2016

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功能：

Factor that induces terminal differentiation of late-developing B-cells to immunoglobulin secreting cells.
亞細胞定位：

Secreted.
蛋白家族：

IL-5 family
數據庫鏈接：

STRING: 10116.ENSRNOP00000010729

UniGene: Rn.44227

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.