Rat Hyaluronic acid,HA ELISA Kit

Rat Hyaluronic acid,HA ELISA Kit

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文獻引用 (13) 說明書下載

中文名稱：

大鼠透明質酸(HA)酶聯免疫試劑盒
貨號：

CSB-E08120r
規格：

96T/48T
價格：

￥3900/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產品描述：

大鼠透明質酸(HA)酶聯免疫試劑盒（CSB-E08120r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的HA含量。靶點研究是醫藥創新的關鍵環節，通過深入疾病分子機制和生物學特性，尋找新的藥物靶點，設計精確治療方案。研究機制包括基因組學、蛋白質組學和生物信息學，以及高通量篩選技術，旨在發現和開發高效、選擇性的藥物。試劑盒檢測范圍為4.69 ng/mL-300 ng/mL，適用于探索HA在組織再生、慢性炎癥或代謝性疾病中的動態變化，如通過檢測肝病模型中的血清HA水平分析纖維化進展，或評估關節炎動物模型中滑膜組織勻漿的HA代謝異常。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

N/A
縮寫：

HA
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

4.69 ng/mL-300 ng/mL
靈敏度：

1.17 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Cell Biology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat HA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	93
	Range %	90-96
1:2	Average %	91
	Range %	87-96
1:4	Average %	97
	Range %	93-100
1:8	Average %	89
	Range %	85-96

回收率：

The recovery of rat HA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	90-96
EDTA plasma (n=4)	95	90-101

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
300	2.656	2.678	2.667	2.505
150	1.848	1.892	1.870	1.708
75	1.202	1.264	1.233	1.071
37.5	0.763	0.778	0.771	0.609
18.75	0.496	0.473	0.485	0.323
9.38	0.384	0.371	0.378	0.216
4.69	0.275	0.298	0.287	0.125
0	0.161	0.163	0.162

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.