Mouse Hyaluronic acid,HA ELISA Kit

Mouse Hyaluronic acid,HA ELISA Kit

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文獻引用 (11) 說明書下載

中文名稱：

小鼠透明質酸(HA)酶聯免疫試劑盒
貨號：

CSB-E08121m
規格：

96T/48T
價格：

￥3800/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產品描述：

小鼠透明質酸(HA)酶聯免疫試劑盒（CSB-E08121m）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的HA含量。HA（血凝素）是流感病毒表面的糖蛋白，在病毒感染宿主細胞過程中起關鍵作用。其可與宿主細胞表面受體結合，介導病毒膜與細胞膜融合。研究HA有助于揭示流感病毒感染機制，為流感疫苗研發、抗病毒藥物設計提供重要靶點。試劑盒檢測范圍為62.5 pg/mL-4000 pg/mL，適用于科研領域對HA表達水平的動態監測。在關節炎模型、皮膚再生研究、腫瘤微環境分析及代謝相關疾病機制探索中具有重要研究價值。適用于比較不同病理狀態下HA分泌水平差異或評估干預措施對HA代謝的影響。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

N/A
縮寫：

HA
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

62.5 pg/mL-4000 pg/mL
靈敏度：

15.6 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Cell Biology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse HA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	89
	Range %	82-99
1:200	Average %	93
	Range %	89-101
1:400	Average %	102
	Range %	98-106
1:800	Average %	97
	Range %	92-102

回收率：

The recovery of mouse HA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	87-94
EDTA plasma (n=4)	97	93-107

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
4000	2.755	2.856	2.806	2.652
2000	2.027	2.128	2.078	1.924
1000	1.435	1.455	1.445	1.291
500	0.850	0.861	0.856	0.702
250	0.526	0.515	0.521	0.367
125	0.358	0.348	0.353	0.199
62.5	0.267	0.261	0.264	0.110
0	0.154	0.153	0.154

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

Targeting hyaluronan synthesis enhances the therapeutic effectiveness of biologics in inflammatory bowel disease P Xiao, Z Chen, X Cai, W Xia, X Liu, Z Song,JCI insight,2025
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A Polysaccharide from Amusium Pleuronectes Combined with Praziquantel Treatment Ameliorates Hepatic Fibrosis in Schistosoma Japonicum-Infected Mice Tang X.et al,Med Sci Monit,2018
Toxoplasma gondii GRA15II effector-induced M1 cells ameliorate liver fibrosis in mice infected with Schistosomiasis japonica. Xie Y.et al,Cell Mol Immunol.,2016
Role of Hematopoietic Stem Cells in Inflammation of the Pancreas during Diabetes Mellitus. Dygai AM.et al,Bull Exp Biol Med.,2016

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.