Rat Gastrin ELISA Kit

Rat Gastrin ELISA Kit

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中文名稱(chēng)：

大鼠胃泌素(Gastrin)酶聯(lián)免疫試劑盒
貨號(hào)：

CSB-E12743r
規(guī)格：

96T/48T
價(jià)格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

產(chǎn)品詳情
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相關(guān)產(chǎn)品
靶點(diǎn)詳情

產(chǎn)品描述：

大鼠胃泌素(Gastrin)酶聯(lián)免疫試劑盒（CSB-E12743r）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織培養(yǎng)上清液,組織勻漿樣本中的GAST含量。GAST作為一種靶點(diǎn)，其背景涉及在生理過(guò)程中發(fā)揮重要作用，如調(diào)節(jié)胃腸道功能等。在研究機(jī)制方面，主要聚焦于其信號(hào)傳導(dǎo)通路，探索其與疾病發(fā)生、發(fā)展的關(guān)聯(lián)，旨在通過(guò)干預(yù)該靶點(diǎn)來(lái)開(kāi)發(fā)針對(duì)相關(guān)疾病的治療策略和藥物。試劑盒檢測(cè)范圍為0.312 pg/mL-20 pg/mL，該產(chǎn)品適用于胃腸功能研究、藥物對(duì)胃泌素分泌影響的評(píng)價(jià)及消化系統(tǒng)疾病模型中激素水平動(dòng)態(tài)分析本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
別名：

Gast ELISA Kit; Gas ELISA Kit; Gastrin [Cleaved into: Big gastrin ELISA Kit; Gastrin-34 ELISA Kit; G34); Gastrin] ELISA Kit
縮寫(xiě)：

GAST
Uniprot No.：

P04563
種屬：

Rattus norvegicus (Rat)
樣本類(lèi)型：

serum, plasma, cell culture supernates ,tissue homogenates
檢測(cè)范圍：

0.312 pg/mL-20 pg/mL
靈敏度：

0.078 pg/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Signal Transduction
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat gastrin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	90
	Range %	85-95
1:200	Average %	94
	Range %	90-98
1:400	Average %	99
	Range %	95-104
1:800	Average %	102
	Range %	98-107

回收率：

The recovery of rat gastrin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	88-96
EDTA plasma (n=4)	100	95-105

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
20	2.496	2.537	2.517	2.390
10	2.181	2.192	2.187	2.060
5	1.795	1.741	1.768	1.641
2.5	1.353	1.313	1.333	1.206
1.25	0.878	0.898	0.888	0.761
0.625	0.369	0.366	0.368	0.241
0.312	0.242	0.259	0.251	0.124
0	0.123	0.130	0.127

數(shù)據(jù)處理：

ELISA操作視頻及標(biāo)曲繪制軟件下載
貨期：

3-5 working days

Proteomic Insights into the Effects of Jianweixiaoshi Tablets on Functional Dyspepsia with Spleen Deficiency in Rats X Cheng, J Wan, D Sun, Y Zhan, J Yu, Y Li,Drug Design, Development and Therapy,2024
Gastroprotective Effects of Spirulina platensis, Golden Kiwifruit Flesh, and Golden Kiwifruit Peel Extracts Individually or in Combination against Indomethacin-Induced Gastric Ulcer in Rats Ibrahim S. Aleid,Nutrients,2021
The Key Ingredient Acacetin in Weishu Decoction Alleviates Gastrointestinal Motility Disorder Based on Network Pharmacology Analysis X Guo,Mediators of Inflammation,2021
Regulation of gastrointestinal hormones during laxative activity of gallotannin-enriched extract isolated from Galla Rhois in loperamide-induced constipation of SD rats Kim JE, et al,Lab Anim Res,2018
Glutinous rice amylopectin can adjust the plasma gut-regulated peptide levels in rhubarb-induced spleen deficiency rats. Zhang H.et al,Food Funct.,2016

功能：

Gastrin stimulates the stomach mucosa to produce and secrete hydrochloric acid and the pancreas to secrete its digestive enzymes. It also stimulates smooth muscle contraction and increases blood circulation and water secretion in the stomach and intestine.
基因功能參考文獻(xiàn)：
1. we examined the chronic effects of a GLP-1-gastrin dual agonist ZP3022 on glycemic control and beta cell dysfunction in overtly diabetic Zucker Diabetic Fatty PMID: 25849341
2. treatment with gastrin, a CCK2R agonist, stimulated the secretion of GLP-1, and that this effect was likely due to increased expression of proglucagon and PCSK1 (also known as prohormone convertase 3 (PC3 gene)). PMID: 25601282
3. The results suggested that clusterin may contribute to gastrin's growth-promoting effect on the oxyntic mucosa. PMID: 21995960
4. plays a role in the development of gastric mucosa, ECL cells and A-like cells in newborn and young rats PMID: 12220729
5. role in increasing expression of COX-2 mRNA and protein, the activity of the COX-2 promoter, and the release of prostaglandin E(2) PMID: 12239223
6. High temperature can affect gastrin, somatostatin and motilin production in the gastric antral mucosa of rats with gastric ulcer. PMID: 12376287
7. study demonstrates that gastrin enhances gastric mucosal integrity through cyclooxygenase-2 PMID: 12388195
8. Data suggest that distention of isolated rat stomach stimulates somatostatin release via cholinergic and non-cholinergic TTX-insensitive pathways, and that these pathways are responsible for distention-induced inhibition of gastrin release. PMID: 12508367
9. gastrin induces Akt through activation of CCKB receptors and of intracellular calcium-dependent, PKC-independent, pathways PMID: 15351698
10. the role of JAK-dependent pathways in the regulation of E-cadherin-dependent cell-cell adhesion by gastrin PMID: 15530849
11. gastrin- and PACAP-stimulated secretion of histamine and pancreastatin is inhibited by somatostatin, misoprostol and galanin in enterochromaffin-like cells PMID: 15935492
12. hypogastrinemia and impaired enterochromaffin cell function in the oxyntic mucosa of the stomach might be partially responsible for the reduction in body weight that occurs after gastric bypass PMID: 16645284
13. G cells are luminal sampling chemomechanosensory cells whose gastrin secretion is regulated by neural, hormonal, luminal, and mechanical factors through protein kinase A activation, cAMP signaling, and mitogen-activated protein kinase phosphorylation. PMID: 19208342
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亞細(xì)胞定位：

Secreted.
蛋白家族：

Gastrin/cholecystokinin family
數(shù)據(jù)庫(kù)鏈接：

KEGG: rno:25320

STRING: 10116.ENSRNOP00000019863

UniGene: Rn.9754

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.