Rat Dehydroepiandrosterone,DHEA ELISA Kit

Rat Dehydroepiandrosterone,DHEA ELISA Kit

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中文名稱：

大鼠脫氫表雄酮(DHEA)酶聯免疫試劑盒
貨號：

CSB-E08227r
規格：

96T/48T
價格：

￥3900/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產品描述：

大鼠脫氫表雄酮(DHEA)酶聯免疫試劑盒（CSB-E08227r）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的DHEA含量。DHEA即脫氫表雄酮，是人體內重要的激素前體。它由腎上腺皮質、性腺及大腦分泌，參與體內多種生理過程。研究機制上，其可調節神經、免疫等系統功能，還與代謝、衰老等密切相關，目前在代謝性疾病、抗衰老等領域有廣泛研究。試劑盒檢測范圍為0.05 ng/mL-10 ng/mL，該產品適用于動物模型中的內分泌研究，如代謝綜合征、多囊卵巢綜合征的機制探索，或用于評估藥物干預對腎上腺功能的影響。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

DHEA
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

0.05 ng/mL-10 ng/mL
靈敏度：

0.025 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Neuroscience
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat DHEA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	102
	Range %	98-107
1:2	Average %	92
	Range %	88-96
1:4	Average %	89
	Range %	85-93
1:8	Average %	100
	Range %	97-104

回收率：

The recovery of rat DHEA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	85-92
EDTA plasma (n=4)	94	88-98

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average
10	0.162	0.158	0.160
2.5	0.307	0.298	0.303
0.8	0.487	0.504	0.496
0.2	0.801	0.810	0.806
0.05	1.224	1.305	1.265
0	2.000	1.899	1.950

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

■ 常見問題解答

Please provide the following information regarding Rat Dehydroepiandrosterone, DHEA ELISA Kit (CSB-E08227r): What is the active ingredient of the stop solution and its concentration?
What is the type, concentration, and state (liquid/lyophilized) of the preservatives used?
What is the expression host of the standard?
which components have H202 and the concentration of H202 in them?Just to confirm this I am inquiring for 24 and 96 well of this kit. Please confirm if the information will be the same for both the 24T and 96T in regards to Rat Dehydroepiandrosterone, DHEA ELISA Kit (CSB-E08227r)?
**if the preservative/s are not liquid, what is the concentration of the component prior to reconstitution (while still lyophilized)?

Thanks for your inquiry.
CSB-E08227r
Standard is purified DHEA.
Stop solution is 4N sulfuric acid.
Preservative is proclin 300(1:3000 dilution）.
Sorry we can not disclose which components have H202 and the concentration of H202 in them.
For 24T and 96T, the component information is the same. Only the volume of reagents is different.
Pls let me know if you have any further questions. Thank you.

Can you please clarify if the preservative is liquid or lyophilized?
If it's lyophilized, please let us know concentration too.

Thanks for your further inquiry. It is liquid.

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Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.