Human Dehydroepiandrosterone,DHEA ELISA Kit

產(chǎn)品詳情

說明書下載

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

人脫氫表雄酮(DHEA)酶聯(lián)免疫試劑盒（CSB-E08226h）為競爭法ELISA試劑盒，定量檢測血清、血漿樣本中的DHEA含量。DHEA即脫氫表雄酮，是體內(nèi)腎上腺皮質(zhì)分泌的一種類固醇激素。它在人體代謝、免疫等方面有重要作用。研究機制主要圍繞其對神經(jīng)、生殖等系統(tǒng)的調(diào)節(jié)，如改善認知功能、影響性激素水平等，相關研究有望為多種疾病防治提供新思路。試劑盒檢測范圍為0.1 ng/mL-20 ng/mL，適用于內(nèi)分泌代謝研究、生殖系統(tǒng)功能評估、衰老機制探索等科研領域，特別適用于激素水平動態(tài)監(jiān)測、藥物干預效果評價等實驗場景。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

DHEA
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma
檢測范圍：

0.1 ng/mL-20 ng/mL
靈敏度：

0.05 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Neuroscience
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human DHEA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	96
	Range %	91-110
1:2	Average %	93
	Range %	87-100
1:4	Average %	95
	Range %	89-104
1:8	Average %	90
	Range %	85-100

回收率：

The recovery of human DHEA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	91-102
EDTA plasma (n=4)	96	92-99

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.