Rat D-Dimer,D2D ELISA Kit

產品詳情

文獻引用 (6) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

大鼠D二聚體(D2D)酶聯免疫試劑盒（CSB-E12984r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養上清液、組織勻漿、細胞裂解物樣本中的D2D含量。大鼠D二聚體（D2D）是纖維蛋白單體交聯后經纖溶酶水解產生的特異性降解產物，是纖溶過程的標記物。其在血栓形成和溶解過程中扮演重要角色，其檢測對于評估血栓性疾病具有重要意義，可用于臨床診斷和科研研究。試劑盒檢測范圍為7.8 ng/mL-500 ng/mL，本產品在凝血功能研究、血栓相關疾病模型建立及藥物作用機制探索中具有重要科研價值，適用于心血管疾病研究、抗凝藥物開發、炎癥反應評估等科研場景。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

D2D
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, cell culture supernates, tissue homogenates, cell lysates
檢測范圍：

7.8 ng/mL-500 ng/mL
靈敏度：

1.95 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Cardiovascular
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat D2D in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	88-96
1:2	Average %	95
	Range %	89-99
1:4	Average %	90
	Range %	86-94
1:8	Average %	102
	Range %	98-106

回收率：

The recovery of rat D2D spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	85	81-92
EDTA plasma (n=4)	89	85-98

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.