Rat Complement fragment 5,C5 ELISA Kit

Rat Complement fragment 5,C5 ELISA Kit

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中文名稱：

大鼠補體片斷5(C5)酶聯(lián)免疫試劑盒
貨號：

CSB-E14363r
規(guī)格：

96T/48T
價格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產(chǎn)品描述：

大鼠補體片斷5(C5)酶聯(lián)免疫試劑盒（CSB-E14363r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的C5含量。C5是補體系統(tǒng)中的關鍵成分，在免疫防御中起重要作用。異常激活會引發(fā)多種疾病。研究機制主要圍繞抑制其激活，以減少炎癥反應和組織損傷。目前針對C5的治療手段，如單抗藥物，在多種病癥中展現(xiàn)出良好應用前景。試劑盒檢測范圍為9.38 ng/mL-600 ng/mL，可應用于大鼠模型中的補體系統(tǒng)功能研究，例如評估炎癥性疾病動物模型的病理進程、藥物干預對補體激活的影響，或探究組織損傷修復過程中C5的動態(tài)變化，為免疫學、藥理學及基礎醫(yī)學研究提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

C5Complement C5 [Cleaved into: C5a anaphylatoxin] ELISA kit; Fragment ELISA kit
縮寫：

C5
Uniprot No.：

P08650
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

9.38 ng/mL-600 ng/mL
靈敏度：

2.34 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat C5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:200	Average %	104
	Range %	97-111
1:400	Average %	101
	Range %	94-108
1:800	Average %	98
	Range %	93-103
1:1600	Average %	91
	Range %	84-97

回收率：

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml

OD1

OD2

Average

Corrected

600

2.218

2.134

2.176

2.070

300

1.864

1.767

1.815

1.710

150

1.346

1.298

1.322

1.216

0.937

0.929

0.933

0.827

37.5

0.601

0.611

0.606

0.500

18.75

0.436

0.453

0.445

0.339

9.38

0.302

0.294

0.298

0.192

0.106

0.105

0.106

數(shù)據(jù)處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

iTRAQ-based proteomic analysis to identify the molecular mechanism of Zhibai Dihuang Granule in the Yin-deficiency-heat syndrome rats Liu CM.et al,Chin Med,2018

功能：

Activation of C5 by a C5 convertase initiates the spontaneous assembly of the late complement components, C5-C9, into the membrane attack complex. C5b has a transient binding site for C6. The C5b-C6 complex is the foundation upon which the lytic complex is assembled.; Derived from proteolytic degradation of complement C5, C5a anaphylatoxin is a mediator of local inflammatory process. Binding to the receptor C5AR1 induces a variety of responses including intracellular calcium release, contraction of smooth muscle, increased vascular permeability, and histamine release from mast cells and basophilic leukocytes. C5a is also a potent chemokine which stimulates the locomotion of polymorphonuclear leukocytes and directs their migration toward sites of inflammation.
亞細胞定位：

Secreted.
數(shù)據(jù)庫鏈接：

STRING: 10116.ENSRNOP00000025534

UniGene: Rn.21259

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.