Rat Collagen Type Ⅳ,Col Ⅳ ELISA KIT

Rat Collagen Type Ⅳ,Col Ⅳ ELISA KIT

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中文名稱：

大鼠Ⅳ型膠原(Col Ⅳ)酶聯(lián)免疫試劑盒
貨號：

CSB-E08883r
規(guī)格：

96T/48T
價格：

￥3900/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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大鼠Ⅳ型膠原(Col Ⅳ)酶聯(lián)免疫試劑盒（CSB-E08883r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的Col Ⅳ含量。Col Ⅳ即Ⅳ型膠原，是基底膜的主要成分。它在維持組織正常結(jié)構(gòu)與功能中發(fā)揮重要作用。研究發(fā)現(xiàn)其異常表達與腫瘤侵襲、轉(zhuǎn)移及多種疾病相關(guān)，機制可能涉及與細胞表面受體相互作用，影響細胞黏附、遷移和信號傳導等過程。試劑盒檢測范圍為1.56 ng/mL-100 ng/mL，可應用于肝/腎纖維化動物模型構(gòu)建、血管基底膜損傷研究、腫瘤微環(huán)境分析等科研領域，特別適用于評估疾病模型中Col Ⅳ的動態(tài)變化及組織損傷修復機制，為心血管疾病、器官纖維化等病理過程的分子機制研究提供精準檢測工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

Collagen Type 4,Col 4,_x000D_ Collagen Type IV,Col IV
縮寫：

Col Ⅳ
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

1.56 ng/mL-100 ng/mL
靈敏度：

0.39 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Signal Transduction
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat COL Ⅳ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	97
	Range %	92-105
1:2	Average %	96
	Range %	92-101
1:4	Average %	92
	Range %	87-96
1:8	Average %	87
	Range %	82-94

回收率：

The recovery of rat COL Ⅳ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	90-98
EDTA plasma (n=4)	104	99-108

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
100	2.221	2.120	2.171	2.019
50	1.441	1.340	1.391	1.239
25	0.825	0.804	0.815	0.663
12.5	0.519	0.530	0.525	0.373
6.25	0.335	0.324	0.330	0.178
3.12	0.240	0.251	0.246	0.094
1.56	0.196	0.192	0.194	0.042
0	0.152	0.151	0.152

數(shù)據(jù)處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.