Human Collagen Type Ⅳ(COL Ⅳ) ELISA Kit

產(chǎn)品詳情

文獻(xiàn)引用 (1) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

人Ⅳ型膠原(Col Ⅳ)酶聯(lián)免疫試劑盒（CSB-E17116h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養(yǎng)上清液、尿液樣本中的Col Ⅳ含量。Col Ⅳ（IV型膠原蛋白）是細(xì)胞基底膜的主要結(jié)構(gòu)成分，參與組織完整性維持和正常功能。其研究機(jī)制涉及細(xì)胞外基質(zhì)組成、細(xì)胞黏附、遷移、生存、擴(kuò)張和分化。在疾病如腫瘤、肝纖維化等中，Col Ⅳ的表達(dá)和功能異常，成為治療研究的重要靶點(diǎn)。試劑盒檢測范圍為62.5 pg/mL-4000 pg/mL，本產(chǎn)品適用于體外研究基底膜相關(guān)疾病機(jī)制、評估藥物對細(xì)胞外基質(zhì)代謝的影響，或通過體液樣本分析Col Ⅳ作為生物標(biāo)志物的潛在價值，為組織修復(fù)、血管生成及纖維化研究提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

Collagen Type 4(COL 4),_x000D_ Collagen Type IV(COL IV)
縮寫：

Col Ⅳ
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, cell culture supernates, urine
檢測范圍：

62.5 pg/mL-4000 pg/mL
靈敏度：

15.6 pg/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Signal Transduction
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human COL Ⅳ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:200	Average %	86
	Range %	80-104
1:400	Average %	97
	Range %	91-105
1:800	Average %	100
	Range %	92-113
1:1600	Average %	93
	Range %	86-98

回收率：

The recovery of human COL Ⅳ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	89-102
EDTA plasma (n=4)	93	85-100

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.