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中文名稱：大鼠Ⅰ型膠原(Col Ⅰ)酶聯免疫試劑盒
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貨號：CSB-E08084r
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規格：96T/48T
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價格：￥3900/￥2500
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促銷：
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其他：
產品詳情
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產品描述：大鼠Ⅰ型膠原(Col Ⅰ)酶聯免疫試劑盒（CSB-E08084r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的Col Ⅰ含量。Col Ⅰ即Ⅰ型膠原蛋白，是細胞外基質的主要成分。其背景在于它廣泛存在于多種組織，對維持組織正常結構和功能起重要作用。研究機制方面，圍繞其在組織纖維化、腫瘤微環境等過程中的合成、降解及信號傳導途徑展開，旨在尋找相關疾病的治療靶點。試劑盒檢測范圍為78 pg/mL-5000 pg/mL，適用于體外實驗環境下對大鼠組織修復機制、纖維化疾病模型評估或骨骼代謝相關研究本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
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別名：Collagen Type 1,Col 1,_x000D_ Collagen Type I,Col I
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縮寫：Col Ⅰ
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種屬：Rattus norvegicus (Rat)
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樣本類型：serum, plasma, tissue homogenates
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檢測范圍：78 pg/mL-5000 pg/mL
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靈敏度：19.5 pg/mL
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反應時間：1-5h
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樣本體積：50-100ul
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檢測波長：450 nm
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研究領域：Signal Transduction
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測定原理：quantitative
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測定方法：Sandwich
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精密度：
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度：
To assess the linearity of the assay, samples were spiked with high concentrations of rat Col Ⅰ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 84 Range % 80-88 1:2 Average % 89 Range % 84-93 1:4 Average % 92 Range % 87-96 1:8 Average % 95 Range % 90-99 -
回收率：
The recovery of rat Col Ⅰ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 94 88-98 EDTA plasma (n=4) 98 93-103 -
標準曲線：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. 
pg/ml OD1 OD2 Average Corrected 5000 2.701 2.762 2.732 2.537 2500 1.903 1.954 1.929 1.734 1250 1.195 1.207 1.201 1.006 625 0.802 0.743 0.773 0.578 312 0.489 0.466 0.478 0.283 156 0.376 0.359 0.368 0.173 78 0.258 0.243 0.251 0.056 0 0.201 0.189 0.195 -
數據處理：
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貨期：3-5 working days
引用文獻
- Chitosan tamarind-based nanoparticles as a promising approach for topical application of curcumin intended for burn healing: in vitro and in vivo study ES Shalaby,Journal of drug targeting,2023
- Silymarin and MSC-exosomes ameliorate thioacetamide-evoked renal fibrosis by inhibiting TGF-β/SMAD pathway in rats DM Mekawy,Molecular biology reports,2024
- Diacerein ameliorates cholestasis-induced liver fibrosis in rat via modulating HMGB1/RAGE/NF-κB/JNK pathway and endoplasmic reticulum stress AM Abdelfattah,Scientific reports,2023
- Cold plasma approach fortifies the topical application of thymoquinone intended for wound healing via up-regulating the levels of TGF-?, VEGF, and α-SMA in rats AA Sedik,International immunopharmacology,2023
- mTOR/ULK1 signaling axis mediated ultrashort wave regulation of autophagy to alleviate diabetic kidney disease Y Yan,Advances in clinical and experimental medicine,2023
- Soluble guanylate cyclase agonist, isoliquiritigenin attenuates renal damage and aortic calcification in a rat model of chronic kidney failure HH Atteia,Life sciences,2023
- Asiaticoside polymeric nanoparticles for effective diabetic wound healing through increased collagen biosynthesis: In-vitro and in-vivo evaluation S Narisepalli,International journal of pharmaceutics,2022
- Risks of Protein Conditioner Application under the Impact of Heat and/or Gamma Irradiation in Albino Rats S Eskande,Arab Journal of Nuclear Sciences and Applications,2021
- Genetic and physiological variation in two strains of Japanese quail NS Ibrahim,J Genet Eng Biotechnol,2021
- Effects of repeated increasing doses of cisplatin as models of acute kidney injury and chronic kidney disease in rats M Al Za'abi,Naunyn-Schmiedeberg's Archives of Pharmacology,2020
- Myrcene exerts anti-asthmatic activity in neonatal rats via modulating the matrix remodeling Y Du,Int J Immunopathol Pharmacol,2020
- miR-16 integrates signal pathways in myofibroblasts: determinant of cell fate necessary for fibrosis resolution Q Pan,Cell Death & Disease,2020
- Novel Long Non-Coding RNA lnc-URIDS Delays Diabetic Wound Healing By Targeting Plod1 M Hu,Diabetes,2020
- Exosomal hsa-miR-21-5p derived from growth hormone-secreting pituitary adenoma promotes abnormal bone formation in acromegaly Yuanyuan Xiong, et al,Translational Research,2019
- Research article Preventive effect of dried plum extract against dexamethasone-induced osteoporosis in male rats through inhibiting cathepsin-K activity, lipogenesis and trabecular bone loss Azza M. El-Wakf,Journal of Innovations in Pharmaceutical and Biological Sciences,2019
- Liraglutide inhibited AGEs induced coronary smooth muscle cell phenotypic transition through inhibiting the NF-κB signal pathway Di B, et al,Peptides,2018
- Modified Xiaochaihu decoction (加味小柴胡湯) promotes collagen degradation and inhibits pancreatic fibrosis in chronic pancreatitis rats Shu-kun Zhang.et al,Chinese Journal of Integrative Medicine,2017
- Multifunctional and biomimetic fish collagen/bioactive glass nanofibers: fabrication, antibacterial activity and inducing skin regeneration in vitro and in vivo Tian Zhou.et al,Int J Nanomedicine,2017
- Placental growth factor and placental protein 13 in patients with Balkan endemic nephropathy, a worldwide disease Stefanovic V. et al,Ren Fail.,2015
- Mechanical stretch promotes matrix metalloproteinase-2 and prolyl-4-hydroxylase a1 production in human aortic smooth muscle cells via Akt-p38 MAPK-JNK signaling. Liu X et al,Int J Biochem Cell Biol,2015
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