Rat Angiopoietin-related protein 4(ANGPTL4) ELISA kit

Rat Angiopoietin-related protein 4(ANGPTL4) ELISA kit

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中文名稱：

大鼠血管生成素相關(guān)蛋白4(ANGPTL4)酶聯(lián)免疫試劑盒
貨號(hào)：

CSB-EL001712RA
規(guī)格：

96T/48T
價(jià)格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產(chǎn)品描述：

大鼠血管生成素相關(guān)蛋白4(ANGPTL4)酶聯(lián)免疫試劑盒（CSB-EL001712RA）檢測(cè)血清、血漿、組織培養(yǎng)上清液、組織勻漿、支氣管肺泡灌洗液、尿液樣本中的ANGPTL4含量。ANGPTL4是一個(gè)重要靶點(diǎn)。它屬于血管生成素樣蛋白家族，在脂質(zhì)代謝、血管生成等生理過程中發(fā)揮作用。研究發(fā)現(xiàn)，它可抑制脂蛋白脂肪酶活性影響甘油三酯代謝。同時(shí)其在腫瘤血管生成等病理機(jī)制中也有參與，為相關(guān)疾病治療提供了潛在方向。試劑盒檢測(cè)范圍為7.8 pg/mL-500 pg/mL，本產(chǎn)品適用于肥胖、糖尿病、動(dòng)脈粥樣硬化等代謝相關(guān)疾病的機(jī)制研究，或炎癥模型、血管新生實(shí)驗(yàn)的分子水平分析，為體外實(shí)驗(yàn)提供高靈敏度的定量檢測(cè)方案本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

Angptl4Angiopoietin-related protein 4 ELISA kit; Angiopoietin-like protein 4 ELISA kit; Hepatic fibrinogen/angiopoietin-related protein ELISA kit; HFARP) [Cleaved into: ANGPTL4 N-terminal chain; ANGPTL4 C-terminal chain] ELISA kit
縮寫：

ANGPTL4
Uniprot No.：

Q6TMA8
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, cell culture supernates, tissue homogenates, bronchoalveolar lavage fluid, urine
檢測(cè)范圍：

7.8 pg/mL-500 pg/mL
靈敏度：

1.95 pg/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Cardiovascular
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat ANGPTL4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:200	Average %	87
Range %	83-102
1:400	Average %	86
Range %	81-90
1:800	Average %	92
Range %	87-98
1:1600	Average %	102
Range %	99-105

回收率：

The recovery of rat ANGPTL4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	88-96
EDTA plasma (n=4)	89	85-93

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
500	2.634	2.687	2.661	2.561
250	2.226	2.268	2.247	2.147
125	1.568	1.534	1.551	1.451
62.5	0.968	0.943	0.956	0.856
31.25	0.527	0.568	0.548	0.448
15.62	0.398	0.377	0.388	0.288
7.8	0.274	0.289	0.282	0.182
0	0.099	0.101	0.100

數(shù)據(jù)處理：

ELISA操作視頻及標(biāo)曲繪制軟件下載
貨期：

3-5 working days

功能：

Mediates inactivation of the lipoprotein lipase LPL, and thereby plays a role in the regulation of triglyceride clearance from the blood serum and in lipid metabolism. May also play a role in regulating glucose homeostasis and insulin sensitivity. Inhibits proliferation, migration, and tubule formation of endothelial cells and reduces vascular leakage. Upon heterologous expression, inhibits the adhesion of endothelial cell to the extracellular matrix (ECM), and inhibits the reorganization of the actin cytoskeleton, formation of actin stress fibers and focal adhesions in endothelial cells that have adhered to ANGPTL4-containing ECM (in vitro). Depending on context, may modulate tumor-related angiogenesis.; Mediates inactivation of the lipoprotein lipase LPL, and thereby plays an important role in the regulation of triglyceride clearance from the blood serum and in lipid metabolism. Has higher activity in LPL inactivation than the uncleaved protein.
基因功能參考文獻(xiàn)：
1. this study represents the first investigation to demonstrate that Angptl4 can predict podocyte injury at earlier stages in minimal change disease PMID: 26352670
2. GR and FoxO1 are required for Angptl4 transcription activation, and that FoxO1 negatively mediates the suppressive effect of insulin PMID: 24565756
3. the overexpression of Angptl4 exerts protective, anti-inflammatory and anti-angiogenic effects. It re-presents a novel therapeutic target gene for the treatment of acute lung injury induced by LPS. PMID: 23783408
4. Data suggest that endothelial lipoprotein lipase (LPL) levels in the heart are altered to maintain FA flux and may exploit angiopoietin-like protein-4 (ANGPTL4). PMID: 22226882
5. Angptl4 is a novel methylation-silenced gene both in rat and human mammary carcinomas. PMID: 21489049
6. Angptl-4 is a fasting-induced controller of lipoprotein lipase in adipose tissue, acting extracellularly on the native conformation in an unusual fashion, like an unfolding molecular chaperone PMID: 17088546
7. ANGPTL4 is a direct GR target that participates in glucocorticoid-regulated triglyceride metabolism. PMID: 19628874
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亞細(xì)胞定位：

Secreted. Secreted, extracellular space, extracellular matrix.
數(shù)據(jù)庫(kù)鏈接：

KEGG: rno:362850

STRING: 10116.ENSRNOP00000010031

UniGene: Rn.119611

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.