Rat Advanced glycosylation end product-specific receptor(AGER) ELISA kit

說明書下載

中文名稱：

大鼠晚期糖基化終產物特異性受體(AGER)酶聯免疫試劑盒
貨號：

CSB-EL001441RA
規格：

96T/48T
價格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產品描述：

大鼠晚期糖基化終產物特異性受體(AGER)酶聯免疫試劑盒（CSB-EL001441RA）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿,組織勻漿樣本中的AGER含量。AGER即晚期糖基化終末產物受體，在炎癥、氧化應激等病理過程發揮重要作用。研究顯示，它可與多種配體結合，激活下游信號通路，引發細胞內一系列反應，參與糖尿病并發癥、神經退行性疾病、腫瘤等疾病發生發展，是潛在治療靶點。試劑盒檢測范圍為31.25 pg/mL-2000 pg/mL，靈敏度為7.81 pg/mL。該產品主要應用于基礎研究領域，如糖尿病相關器官損傷的分子機制探索、神經炎癥模型的蛋白表達分析，或藥物干預下AGER信號通路變化的體外評估，為科研人員提供可靠的實驗工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

Ager ELISA Kit; Rage ELISA Kit; Advanced glycosylation end product-specific receptor ELISA Kit; Receptor for advanced glycosylation end products ELISA Kit
縮寫：

AGER
Uniprot No.：

Q63495
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma,tissue homogenates
檢測范圍：

31.25 pg/mL-2000 pg/mL
靈敏度：

7.81 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean(pg/ml)	224.423	224.423	225.339	224.377	225.431	224.881
SD	0.034	0.030	0.031	0.040	0.037	0.039
CV(%)	4.433	3.911	4.031	5.216	4.810	5.078

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat AGER in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

Sample

Serum(n=4)

1:1

Average %

Range %

90-94

1:2

Average %

Range %

90-102

1:4

Average %

102

Range %

99-105

1:8

Average %

Range %

80-94

回收率：

The recovery of rat AGER spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	87-95
EDTA plasma (n=4)	101	97-106

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml

OD1

OD2

Average

Corrected

2000

2.703

2.878

2.791

2.615

1000

1.903

1.856

1.880

1.704

500

1.245

1.154

1.200

1.024

250

0.731

0.747

0.739

0.563

125

0.556

0.533

0.545

0.369

62.5

0.402

0.392

0.397

0.221

31.25

0.272

0.275

0.274

0.098

0.173

0.179

0.176

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

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最新研究進展：

最新研究發現，AGER基因的多態性可能與各種疾病的發生有關，包括糖尿病、肺炎、慢性阻塞性肺病等。此外，AGER的表達也與肺癌、乳腺癌、胃癌等腫瘤的發生和發展有關。
功能：

Mediates interactions of advanced glycosylation end products (AGE). These are nonenzymatically glycosylated proteins which accumulate in vascular tissue in aging and at an accelerated rate in diabetes. Acts as a mediator of both acute and chronic vascular inflammation in conditions such as atherosclerosis and in particular as a complication of diabetes. AGE/RAGE signaling plays an important role in regulating the production/expression of TNF-alpha, oxidative stress, and endothelial dysfunction in type 2 diabetes. Interaction with S100A12 on endothelium, mononuclear phagocytes, and lymphocytes triggers cellular activation, with generation of key proinflammatory mediators. Receptor for amyloid beta peptide. Contributes to the translocation of amyloid-beta peptide (ABPP) across the cell membrane from the extracellular to the intracellular space in cortical neurons. ABPP-initiated RAGE signaling, especially stimulation of p38 mitogen-activated protein kinase (MAPK), has the capacity to drive a transport system delivering ABPP as a complex with RAGE to the intraneuronal space. Can also bind oligonucleotides. Interaction with S100B after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling.
亞細胞定位：

Membrane; Single-pass type I membrane protein.
組織特異性：

Endothelial cells and cardiomyocytes.
數據庫鏈接：

STRING: 10116.ENSRNOP00000000508

UniGene: Rn.9829

Rat Advanced glycosylation end product-specific receptor(AGER) ELISA kit

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