Rabbit procollagen Ⅰ N-terminal peptide(PⅠNP)ELISA Kit

產品詳情

文獻引用 (3) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

兔子Ⅰ型前膠原N端前肽(PⅠNP)酶聯免疫試劑盒（CSB-E17085Rb）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的PⅠNP含量。PⅠNP是一種重要靶點。它與骨代謝密切相關，是I型前膠原氨基端前肽。研究其機制主要聚焦在它反映成骨細胞活性和骨形成情況的作用上，通過檢測它可輔助診斷骨質疏松癥等骨病，在骨健康研究和臨床診斷中有重要意義。試劑盒檢測范圍為0.78 ng/mL-50 ng/mL，該產品可廣泛應用于骨代謝機制研究、骨骼疾病動物模型評估、骨再生藥物開發等科研領域，為骨生物學、骨組織工程及代謝性骨病的實驗研究提供可靠數據支持。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

procollagen 1 N-terminal peptide(P1NP),_x000D_ procollagen I N-terminal peptide(PINP)
縮寫：

PⅠNP
種屬：

Oryctolagus cuniculus (Rabbit)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

0.78 ng/mL-50 ng/mL
靈敏度：

0.195 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Others
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit PⅠNP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	84
	Range %	80-92
1:2	Average %	96
	Range %	91-105
1:4	Average %	103
	Range %	92-110
1:8	Average %	93
	Range %	84-98

回收率：

The recovery of rabbit PⅠNP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-98
EDTA plasma (n=4)	89	82-96

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.