Rabbit Soluble protein-100B,S-100B ELISA Kit

產品詳情

產品描述：

兔子S100B蛋白(S-100B)酶聯免疫試劑盒（CSB-E06895Rb）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的S100B含量。S100B是一種神經膠質細胞源性鈣結合蛋白，在神經系統中發揮多種作用。其與神經發育、損傷修復等過程密切相關。研究機制主要聚焦于其參與神經細胞內信號傳導、調節神經細胞的存活和凋亡等方面，為神經相關疾病的研究提供了重要靶點。試劑盒檢測范圍為31.25 pg/mL-2000 pg/mL，支持科研人員高效分析不同樣本中S100B的濃度動態，適用于神經退行性疾病模型研究、腦損傷動物實驗、神經膠質瘤機制探索等基礎研究領域。為實驗室開展神經生物學、細胞應激反應等研究提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

S100B ELISA Kit; Protein S100-B ELISA Kit; S-100 protein beta chain ELISA Kit; S-100 protein subunit beta ELISA Kit; S100 calcium-binding protein B ELISA Kit
縮寫：

S100B
Uniprot No.：

Q6YNR6
種屬：

Oryctolagus cuniculus (Rabbit)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

31.25 pg/mL-2000 pg/mL
靈敏度：

7.8 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Signal Transduction
測定原理：

quantitative
測定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit S-100B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	88
	Range %	84-92
1:2	Average %	94
	Range %	90-99
1:4	Average %	103
	Range %	99-110
1:8	Average %	93
	Range %	89-98

回收率：

The recovery of rabbit S-100B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	90-100
EDTA plasma (n=4)	97	92-104

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Weakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites. Binds to and initiates the activation of STK38 by releasing autoinhibitory intramolecular interactions within the kinase. Interaction with AGER after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling. Could assist ATAD3A cytoplasmic processing, preventing aggregation and favoring mitochondrial localization. May mediate calcium-dependent regulation on many physiological processes by interacting with other proteins, such as TPR-containing proteins, and modulating their activity.
基因功能參考文獻：
1. The time course of S100B serum values following spinal cord decompression correlates with outcome; the initial degree of paresis is not a prognostic factor to predict outcome. PMID: 21535942
亞細胞定位：

Cytoplasm. Nucleus.
蛋白家族：

S-100 family
數據庫鏈接：

KEGG: ocu:100009495

STRING: 9986.ENSOCUP00000001267

UniGene: Ocu.2593