Rabbit Prostaglandin E2,PG-E2 ELISA Kit

產(chǎn)品詳情

說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

兔子前列腺素E2(PGE2)酶聯(lián)免疫試劑盒（CSB-E06960Rb）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿、細(xì)胞裂解物樣本中的PG-E2含量。PG - E2即前列腺素 E2，是一種具有廣泛生理和病理作用的脂質(zhì)介質(zhì)。它參與炎癥反應(yīng)、免疫調(diào)節(jié)、細(xì)胞增殖等多種生理過程。在研究機(jī)制上，通過與特定受體結(jié)合激活下游信號(hào)通路，在腫瘤、炎癥性疾病等領(lǐng)域是熱點(diǎn)研究對(duì)象。試劑盒檢測(cè)范圍為31.25 pg/mL-2000 pg/mL，可廣泛應(yīng)用于實(shí)驗(yàn)動(dòng)物模型中炎癥動(dòng)態(tài)監(jiān)測(cè)、藥理學(xué)研究中的化合物效能評(píng)估，以及體外細(xì)胞實(shí)驗(yàn)中炎癥因子調(diào)控機(jī)制的探索本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

PG-E2
種屬：

Oryctolagus cuniculus (Rabbit)
樣本類型：

serum, plasma, tissue homogenates, cell lysates
檢測(cè)范圍：

31.25 pg/mL-2000 pg/mL
靈敏度：

7.81 pg/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Signal Transduction
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit PG-E2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	90
	Range %	80-98
1:2	Average %	96
	Range %	91-100
1:4	Average %	94
	Range %	90-105
1:8	Average %	97
	Range %	86-98

回收率：

The recovery of rabbit PG-E2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	85-110
EDTA plasma (n=4)	90	80-98

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.