Rabbit Parathyroid hormone,PTH ELISA Kit

產(chǎn)品詳情

說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

兔甲狀旁腺激素(PTH)酶聯(lián)免疫試劑盒（CSB-E13244Rb）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿樣本中的PTH含量。甲狀旁腺激素（PTH）是甲狀旁腺主細(xì)胞分泌的肽類激素，主要作用是調(diào)節(jié)鈣磷代謝，維持血鈣平衡。研究機(jī)制方面，PTH可作用于骨骼、腎臟等靶器官，促進(jìn)骨鈣釋放、增加腎小管對(duì)鈣重吸收等，其異常與多種鈣磷代謝相關(guān)疾病密切相關(guān)。試劑盒檢測范圍為7.8 pg/mL-500 pg/mL，可應(yīng)用于內(nèi)分泌研究、鈣代謝調(diào)節(jié)機(jī)制探索、動(dòng)物疾病模型（如甲狀旁腺功能異常）的激素水平評(píng)估等科研場景，為實(shí)驗(yàn)動(dòng)物生理狀態(tài)監(jiān)測及藥物干預(yù)效果評(píng)價(jià)提供可靠數(shù)據(jù)支持。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

PTH
Uniprot No.：

G1SNZ6
種屬：

Oryctolagus cuniculus (Rabbit)
樣本類型：

serum, plasma
檢測范圍：

7.8 pg/mL-500 pg/mL
靈敏度：

1.95 pg/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Signal Transduction
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit PTH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	86-96
1:2	Average %	98
	Range %	93-104
1:4	Average %	91
	Range %	86-97
1:8	Average %	89
	Range %	87-92

回收率：

The recovery of rabbit PTH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	87-94
EDTA plasma (n=4)	97	92-101

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.